ATP Photosynthetic vesicles for light-driven bioprocesses
ATP Photosynthetic vesicles for light-driven bioprocesses
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DOI：
10.1007/s10529-011-0544-5
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发表时间：
2011-06-01
影响因子：
2.7
通讯作者：
Kino, Kuniki
中科院分区：
文献类型：
--
作者：
Hara, Kiyotaka Y.;Suzuki, Rie;Kino, Kuniki
我们准备ATP光合囊泡从内而外的大肠杆菌细胞膜表达δ-视紫红质（一种新的光驱动H？转运蛋白）和TF 0 F1-ATP合酶（一种热稳定性ATP合酶）。这些囊泡表现出光依赖性ATP合成。此外，耦合的ATP光合囊泡与ATP水解己糖激酶启用光依赖性的葡萄糖消耗。ATP光合囊泡在光驱动ATP再生生物过程中有着广泛的应用前景。
We prepared ATP photosynthetic vesicles from inside-out membranes of Escherichia coli cells that express delta-rhodopsin (a novel light-driven H ? transporter) and TF0F1-ATP synthase (a thermostable ATP synthase). These vesicles showed light-dependent ATP synthesis. Furthermore, coupling the ATP photosynthetic vesicles with an ATP-hydrolyzing hexokinase enabled light-dependent glucose consumption. The ATP photosynthetic vesicles indicate their potential to applied to light-driven ATP-regenerating bioprocess for various ATP-hydrolyzing bioproductions.