Inner Envelope CHLOROPLAST MANGANESE TRANSPORTER 1 Supports Manganese Homeostasis and Phototrophic Growth in Arabidopsis
Inner Envelope CHLOROPLAST MANGANESE TRANSPORTER 1 Supports Manganese Homeostasis and Phototrophic Growth in Arabidopsis
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内膜叶绿体锰转运蛋白 1 支持拟南芥的锰稳态和光养生长
DOI：
10.1016/j.molp.2018.04.007
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发表时间：
2018
期刊：
影响因子：
27.5
通讯作者：
Luan S
中科院分区：
文献类型：
--
作者：
Zhang Bin;Zhang Chi;Liu Congge;Jing Yanping;Wang Yuan;Jin Ling;Yang Lei;Zhao Fugeng;Lan Wenzhi;Zhang Bin;Zhang Chi;Fu Aigen;Shi Jisen;Luan Sheng;Lan WZ;Luan S
锰（Mn）是Mn簇中的重要催化金属，其在光合作用期间氧化水以产生氧气。然而，负责Mn 2+输入到叶绿体的转运蛋白仍然未知。在此，我们报道了非特征蛋白家族0016（UPF 0016）中一个进化上保守的蛋白质叶绿体锰转运蛋白1（Chloroplast Manganese Transporter 1，CMT 1）的特性，该蛋白主要在绿色组织中表达，其编码产物定位于叶绿体的内被膜。CMT 1在T-DNA插入突变体cmt 1 - 1中的破坏导致植物生长发育迟缓，类囊体堆积缺陷，光系统II复合物和光合活性严重降低。与放氧能力降低相一致，突变体叶绿体中锰含量低于野生型。CMT 1蛋白作为锰转运蛋白的功能支持了Mn 2+摄取缺陷突变体（Δ smf 1）在酵母细胞中的生长和Mn 2+积累。综上所述，我们的研究结果表明，CMT 1功能作为一个内包膜锰转运负责叶绿体Mn 2+的吸收。
Manganese (Mn) is an essential catalytic metal in the Mn-cluster that oxidizes water to produce oxygen during photosynthesis. However, the transport protein(s) responsible for Mn2+import into the chloroplast remains unknown. Here, we report the characterization ofArabidopsisCMT1 (Chloroplast Manganese Transporter 1), an evolutionarily conserved protein in the Uncharacterized Protein Family 0016 (UPF0016), that is required for manganese accumulation into the chloroplast.CMT1is expressed primarily in green tissues, and its encoded product is localized in the inner envelope membrane of the chloroplast. Disruption ofCMT1in the T-DNA insertional mutantcmt1-1resulted in stunted plant growth, defective thylakoid stacking, and severe reduction of photosystem II complexes and photosynthetic activity. Consistent with reduced oxygen evolution capacity, the mutant chloroplasts contained less manganese than the wild-type ones. In support of its function as a Mn transporter, CMT1 protein supported the growth and enabled Mn2+accumulation in the yeast cells of Mn2+-uptake deficient mutant (Δsmf1). Taken together, our results indicate that CMT1 functions as an inner envelope Mn transporter responsible for chloroplast Mn2+uptake.