Comparative Mapping and Targeted-Capture Sequencing of the Gametocidal Loci in Aegilops sharonensis
Comparative Mapping and Targeted-Capture Sequencing of the Gametocidal Loci in Aegilops sharonensis
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DOI：
10.3835/plantgenome2016.09.0090
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发表时间：
2017-07-01
期刊：
影响因子：
4.2
通讯作者：
King, Julie
中科院分区：
文献类型：
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作者：
Grewal, Surbhi;Gardiner, Laura-Jayne;King, Julie
当引入小麦（小麦L.）时，沙融山羊草（Aegilops sharonensis Eig）等物种中的杀配子（Gc）染色体或元件优先通过雄性和雌性配子传递给下一代。此外，任何基因，如控制农艺学上重要性状的基因，显示出与Gc元件的完全连锁，也优先传递到下一代，而不需要选择。Gc元件优先传递的机制似乎是通过在任何缺乏Gc染色体的配子中诱导广泛的染色体损伤而发生的。以往的研究Gc的作用机制在Ae。sharonensis表明至少涉及两个连锁元件。第一个是断裂元件，它在已经失去Gc元件的配子中诱导染色体断裂，而第二个是抑制元件，它在携带Gc元件的配子中阻止断裂元件的染色体断裂作用。在这项研究中，我们已经使用比较基因组研究地图54个单核苷酸多态性（SNP）标记在一个Ae。sharonensis4SshL渐渗片段，并在小麦中鉴定了18个候选基因。通过对该4SshL基因渗入片段的靶向测序，将该基因渗入片段与sharonensis中的breaker元件进行比较。这一有价值的基因组资源将有助于进一步绘制Gc基因座的图谱，该基因座可用于小麦育种以产生新的、上级小麦品种。
Gametocidal (Gc) chromosomes or elements in species such as Aegilops sharonensis Eig are preferentially transmitted to the next generation through both the male and female gametes when introduced into wheat (Triticum aestivum L.). Furthermore, any genes, such as genes that control agronomically important traits, showing complete linkage with Gc elements, are also transmitted preferentially to the next generation without the need for selection. The mechanism for the preferential transmission of the Gc elements appears to occur by the induction of extensive chromosome damage in any gametes that lack the Gc chromosome in question. Previous studies on the mechanism of the Gc action in Ae. sharonensis indicates that at least two linked elements are involved. The first, the breaker element, induces chromosome breakage in gametes, which have lost the Gc elements while the second, the inhibitor element, prevents the chromosome breakage action of the breaker element in gametes which carry the Gc elements. In this study, we have used comparative genomic studies to map 54 single nucleotide polymorphism (SNP) markers in an Ae. sharonensis 4SshL introgression segment in wheat and have also identified 18 candidate genes in Ae. sharonensis for the breaker element through targeted sequencing of this 4SshL introgression segment. This valuable genomic resource will aide in further mapping the Gc locus that could be exploited in wheat breeding to produce new, superior varieties of wheat.