High-speed multicolor structured illumination microscopy using a hexagonal single mode fiber array
High-speed multicolor structured illumination microscopy using a hexagonal single mode fiber array
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DOI：
10.1364/boe.416546
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发表时间：
2021-02-01
影响因子：
3.4
通讯作者：
Rieger, Bernd
中科院分区：
文献类型：
--
作者：
Hinsdale, Taylor A.;Stallinga, Sjoerd;Rieger, Bernd
结构照明显微镜（SIM）是一种广泛使用的成像技术，其有效分辨率是宽视场显微镜的两倍。大多数当前的实现依赖于衍射元件，光栅或可编程器件，以在样品中生成结构化光图案。这些可能受到频谱效率、速度或两者的限制。在这里，我们介绍了光纤SIM的概念，它允许在宽波长范围内进行相机帧速率受限的图案生成和操作。通过将激光束耦合到六边形单模光纤阵列中的径向相对的光纤对中来产生照明图案，其中出射光束被中继到显微镜物镜的后焦平面。照明图案的相位步进和旋转由快速电光装置控制。我们实现了每秒111 SIM帧的速率，并用488 nm和532 nm激光器产生的激发图案成像。(C)根据OSA开放获取出版协议的条款，2021年美国光学学会
Structured illumination microscopy (SIM) is a widely used imaging technique that doubles the effective resolution of widefield microscopes. Most current implementations rely on diffractive elements, either gratings or programmable devices, to generate structured light patterns in the sample. These can be limited by spectral efficiency, speed, or both. Here we introduce the concept of fiber SIM that allows for camera frame rate limited pattern generation and manipulation over a broad wavelength range. Illumination patterns are generated by coupling laser beams into radially opposite pairs of fibers in a hexagonal single mode fiber array where the exit beams are relayed to the microscope objective's back focal plane. The phase stepping and rotation of the illumination patterns are controlled by fast electro-optic devices. We achieved a rate of 111 SIM frames per second and imaged with excitation patterns generated by both 488 nm and 532 nm lasers. (C) 2021 Optical Society of America under the terms of the OSA Open Access Publishing Agreement