Adhesion of uropathogenic Escherichia coli to epithelial cells from women with recurrent urinary tract infection.
Adhesion of uropathogenic Escherichia coli to epithelial cells from women with recurrent urinary tract infection.
复制标题
尿路致病性大肠杆菌粘附在患有复发性尿路感染的女性的上皮细胞上。
DOI：
10.1007/bf01640776
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发表时间：
1982
期刊：
影响因子：
7.5
通讯作者：
Falkowski,WS
中科院分区：
文献类型：
--
作者：
Schaeffer,AJ;Jones,JM;Duncan,JL;Chmiel,JS;Plotkin,BJ;Falkowski,WS
本文研究了大肠杆菌对取自健康妇女中段尿的人尿路上皮细胞、取自11名健康妇女和24名前一年至少有三次尿路感染的病人的阴道和口腔细胞的粘附性。使用[3H]尿苷标记的细菌，通过聚碳酸酯膜滤器（5 μ m孔径）真空过滤，将未附着的微生物与上皮细胞分离。注意到尿路上皮细胞的感受性的逐日变化。患者的阴道和口腔细胞粘附性变化的范围和速度均大于对照组。患者对阴道细胞的粘附性高于对照组（每个细胞10.1±0.92个细菌vs. 3.8±0.47个细菌[平均值± S. E.]，与颊上皮细胞粘附率（11.7±1.29 vs.7.1 ±0.49，P= 0.002）比较，差异有统计学意义（P<0.001）。阴道和口腔细胞感受性之间存在非常强的非线性正相关（R=0.87，P<0.0001）。这些数据表明，女性尿路感染的易感性与上皮细胞粘附特性的广泛波动变化有关。
Adherence ofEscherichia colito human uroepithelial cells obtained from the midstream urine of healthy women, and to vaginal and buccal cells obtained from 11 healthy women and 24 patients who had had at least three urinary tract infections in the preceding year was studied. Bacteria labeled with [3H] uridine were used, and unattached organisms were separated from the epithelial cells by vacuum filtration through a polycarbonate membrane filter (5-µm-pore-size). A day-to-day variation in the receptivity of uroepithelial cells was noted. The range and rapidity of change in adherence to both vaginal and buccal cells were greater in patients than in controls. Adherence to vaginal cells was greater in patients than in controls (10.1±0.92 vs. 3.8±0.47 bacteria per cell [mean ± S. E.], P<0.001), as was adherence to buccal cells (11.7±1.29 vs. 7.1±0.49, P=0.002). There was a very strong, positive non-linear correlation between vaginal and buccal cell receptivity (R=0.87, P<0.0001). The data suggest that susceptibility in women to urinary-tract infections is associated with widespread, fluctuating changes in the adhesive characteristics of epithelial cells.