The Human Glioblastoma Cell Culture Resource: Validated Cell Models Representing All Molecular Subtypes.
The Human Glioblastoma Cell Culture Resource: Validated Cell Models Representing All Molecular Subtypes.
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DOI：
10.1016/j.ebiom.2015.08.026
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发表时间：
2015-10
期刊：
影响因子：
11.1
通讯作者：
Uhrbom L
中科院分区：
文献类型：
--
作者：
Xie Y;Bergström T;Jiang Y;Johansson P;Marinescu VD;Lindberg N;Segerman A;Wicher G;Niklasson M;Baskaran S;Sreedharan S;Everlien I;Kastemar M;Hermansson A;Elfineh L;Libard S;Holland EC;Hesselager G;Alafuzoff I;Westermark B;Nelander S;Forsberg-Nilsson K;Uhrbom L
胶质母细胞瘤（GBM）是原发性脑肿瘤中最常见和最恶性的形式。GBM基本上是不可治愈的，其对治疗的抗性归因于称为胶质瘤干细胞（GSC）的细胞亚群。为了满足目前相关GBM细胞（GC）系的短缺，我们开发了来源于GBM患者的手术样品的经注释和验证的细胞系的文库，其维持在保留GSC特征的条件下。这个集合，我们称之为人类胶质母细胞瘤细胞培养（HGCC）资源，由48个GC系的生物库和包含高分辨率分子数据的相关数据库组成。我们证明了HGCC系是致瘤的，具有GBM的基因组病变特征，并代表了所有四种转录亚型。HGCC小组为大部分GBM多样性的体外和体内建模提供了一个开放的资源，对基础和翻译GBM研究都很有用。HGCC资源包含48个注释的人GBM细胞系和一个交互式数据库GBM细胞系在干细胞条件下繁殖并显示GSC特征HGCC资源提供所有分子（TCGA）亚型的细胞系与HGCC细胞系相关的所有数据可以在www.example.com上访问
Glioblastoma (GBM) is the most frequent and malignant form of primary brain tumor. GBM is essentially incurable and its resistance to therapy is attributed to a subpopulation of cells called glioma stem cells (GSCs). To meet the present shortage of relevant GBM cell (GC) lines we developed a library of annotated and validated cell lines derived from surgical samples of GBM patients, maintained under conditions to preserve GSC characteristics. This collection, which we call the Human Glioblastoma Cell Culture (HGCC) resource, consists of a biobank of 48 GC lines and an associated database containing high-resolution molecular data. We demonstrate that the HGCC lines are tumorigenic, harbor genomic lesions characteristic of GBMs, and represent all four transcriptional subtypes. The HGCC panel provides an open resource for in vitro and in vivo modeling of a large part of GBM diversity useful to both basic and translational GBM research. The HGCC resource contains 48 annotated human GBM cell lines and an interactive database The GBM cell lines are propagated in stem cell conditions and display GSC characteristics The HGCC resource provides cell lines of all molecular (TCGA) subtypes All data connected with the HGCC cell lines can be accessed at hgcc.se