Interaction of Globular Albumins with the Silica Surface
Interaction of Globular Albumins with the Silica Surface
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DOI：
10.1023/a:1010453409208
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发表时间：
2001-03
影响因子：
1
通讯作者：
Y. Tarasevich
中科院分区：
文献类型：
--
作者：
Y. Tarasevich
牛血清白蛋白大分子与二氧化硅表面羰基处的邻位羟基相互作用，并与酰亚胺基团处的孤立表面羟基相互作用。相对于白蛋白吸附，孪晶表面羟基表现为单一吸附位点。对石英表面上的这些基团的浓度进行了评价。球形白蛋白的二级结构由于二氧化硅-球形白蛋白系统中的相互作用而改变。表面β-链段扩展，α-螺旋浓度降低。这种改变导致白蛋白的三级球状结构发生变化。白蛋白大分子在二氧化硅表面的吸附是倾斜的。
Bovine serum albumin macromolecules interact with vicinal hydroxyl groups of the silica surface at carbonyl groups and with isolated surface hydroxyl groups at the imide groups. Geminal surface hydroxyl groups behave as single adsorption sites relative to albumin adsorption. The concentration of such groups on a quartz surface was evaluated. The secondary structure of globular albumins is altered as the result of interaction in the silica–globular albumin system. Extended β-segments appear on the surface and the concentration of α-spirals decreases. This alteration leads to change in the tertiary globular structure of the albumin. The adsorption of albumin macromolecules on the silica surface is inclined.