Global gene expression changes in Candidatus Liberibacter asiaticus during the transmission in distinct hosts between plant and insect.
Global gene expression changes in Candidatus Liberibacter asiaticus during the transmission in distinct hosts between plant and insect.
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DOI：
10.1111/mpp.12015
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发表时间：
2013-05
影响因子：
4.9
通讯作者：
Qing Yan;A. Sreedharan;Shiping Wei;Jihua Wang;K. Pelz-Stelinski;S. Folimonova;Nian Wang
中科院分区：
文献类型：
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作者：
Qing Yan;A. Sreedharan;Shiping Wei;Jihua Wang;K. Pelz-Stelinski;S. Folimonova;Nian Wang
黄龙病是一种世界性的柑橘毁灭性病害，与柑桔黄龙病菌（Citrus Liberibacter asiaticus）有关。这种韧皮部限制的苛养病原体由亚洲柑橘木虱（Diaphorina citri）传播，并且似乎是一种细胞内病原体，在其整个生命周期中与木虱或植物保持密切联系。这种病原体与其宿主相互作用的分子基础还不清楚。我们假设，在感染期间，Ca。L. asiaticus在任一宿主中差异表达对其存活和/或致病性至关重要的基因。为了验证这一假设，进行定量逆转录聚合酶链反应，以比较钙的基因表达。L.在植物和木虱中的asiaticus。总之，分析了381个基因在植物和木虱中的基因表达。其中182个基因在植物体中的表达量显著高于木虱体（P < 0.05），16个基因在木虱体中的表达量显著高于木虱体（P < 0.05），183个基因在植物体和木虱体中的表达量差异不显著（P ≥ 0.05）。我们的研究表明Ca. L.为了适应植物和昆虫的不同环境，亚洲虎的转录调控、运输系统、分泌系统、鞭毛组装、代谢途径和抗逆性等基因发生了显著的宿主特异性变化。据我们所知，这是第一个大规模的研究，以评估钙的差异表达。L.本发明公开了一种植物宿主及其昆虫载体中的亚洲蝽基因。
Huanglongbing (HLB) or citrus greening disease is a destructive disease of citrus worldwide, which is associated with Candidatus Liberibacter asiaticus. This phloem-limited fastidious pathogen is transmitted by the Asian citrus psyllid, Diaphorina citri, and appears to be an intracellular pathogen that maintains an intimate association with the psyllid or the plant throughout its life cycle. The molecular basis of the interaction of this pathogen with its hosts is not well understood. We hypothesized that, during infection, Ca. L. asiaticus differentially expresses the genes critical for its survival and/or pathogenicity in either host. To test this hypothesis, quantitative reverse transcription-polymerase chain reaction was performed to compare the gene expression of Ca. L. asiaticus in planta and in psyllid. Overall, 381 genes were analysed for their gene expression in planta and in psyllid. Among them, 182 genes were up-regulated in planta compared with in psyllid (P < 0.05), 16 genes were up-regulated in psyllid (P < 0.05) and 183 genes showed no statistically significant difference (P ≥ 0.05) in expression between in planta and in psyllid. Our study indicates that the expression of the Ca. L. asiaticus genes involved in transcriptional regulation, transport system, secretion system, flagella assembly, metabolic pathway and stress resistance are changed significantly in a host-specific manner to adapt to the distinct environments of plant and insect. To our knowledge, this is the first large-scale study to evaluate the differential expression of Ca. L. asiaticus genes in a plant host and its insect vector.