A photoactivatable push-pull fluorophore for single-molecule imaging in live cells
A photoactivatable push-pull fluorophore for single-molecule imaging in live cells
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DOI：
10.1021/ja802883k
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发表时间：
2008-07-23
影响因子：
15
通讯作者：
Moerner, W. E.
中科院分区：
文献类型：
--
作者：
Lord, Samuel J.;Conley, Nicholas R.;Moerner, W. E.
我们已经重新设计了一个红色发光的二氰基亚甲基二氢呋喃推拉荧光团，使其在短时间内被低强度紫光光激活之前是黑暗的。暗荧光团的光活化导致叠氮化物转化为胺，这将吸收转移到长波长。在光活化后，荧光团是明亮的，光稳定的，足以在活细胞中的单分子水平上成像。这种原理验证演示提供了一类新的明亮的光活化荧光团，这是需要主动控制单分子发射的超分辨率成像方案所需要的。
We have reengineered a red-emitting dicyanomethylenedihydrofuran push-pull fluorophore so that it is dark until photoactivated with a short burst of low-intensity violet light. Photoactivation of the dark fluorogen leads to conversion of an azide to an amine, which shifts the absorption to long wavelengths. After photoactivation, the fluorophore is bright and photostable enough to be imaged on the single-molecule level in living cells. This proof-of-principle demonstration provides a new class of bright photoactivatable fluorophores, as are needed for super-resolution imaging schemes that require active control of single molecule emission.