Accurate color imaging of pathology slides using holography and absorbance spectrum estimation of histochemical stains.
Accurate color imaging of pathology slides using holography and absorbance spectrum estimation of histochemical stains.
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使用全息术和组织化学染色的吸收光谱估计对病理切片进行准确的彩色成像。
DOI：
10.1002/jbio.201800335
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发表时间：
2019
影响因子：
2.8
通讯作者：
Ozcan,Aydogan
中科院分区：
文献类型：
--
作者：
Zhang,Yibo;Liu,Tairan;Huang,Yujia;Teng,Da;Bian,Yinxu;Wu,Yichen;Rivenson,Yair;Feizi,Alborz;Ozcan,Aydogan
由于使用窄带照明源，全息显微镜对颜色再现提出了挑战，这特别影响了用于临床诊断的染色病理学载玻片的成像。在这里，一个准确的彩色全息显微镜框架，使用吸收光谱估计。该方法使用以小数目（例如，三到六个）波长，估计样品的吸收光谱，并将其投影到彩色三色刺激上。使用该方法，波长选择被优化以全息成像具有不同组织和染色剂组合的25个病理载玻片样本，从而显著减少最终重建图像中的颜色误差。结果可以被用来作为一个实际的指导各种成像应用，特别是，以纠正在全息成像的病理样本跨越不同的染料和组织类型的颜色失真。
Holographic microscopy presents challenges for color reproduction due to the usage of narrow‐band illumination sources, which especially impacts the imaging of stained pathology slides for clinical diagnoses. Here, an accurate color holographic microscopy framework using absorbance spectrum estimation is presented. This method uses multispectral holographic images acquired and reconstructed at a small number (e.g., three to six) of wavelengths, estimates the absorbance spectrum of the sample, and projects it onto a color tristimulus. Using this method, the wavelength selection is optimized to holographically image 25 pathology slide samples with different tissue and stain combinations to significantly reduce color errors in the final reconstructed images. The results can be used as a practical guide for various imaging applications and, in particular, to correct color distortions in holographic imaging of pathology samples spanning different dyes and tissue types.