N-(9-acridinyl)-bromoacetamide--a powerful reagent for phase-transfer-catalyzed fluorescence labeling of carboxylic acids for liquid chromatography.
N-(9-acridinyl)-bromoacetamide--a powerful reagent for phase-transfer-catalyzed fluorescence labeling of carboxylic acids for liquid chromatography.
复制标题
N-(9-吖啶基)-溴乙酰胺——一种用于液相色谱相转移催化荧光标记羧酸的强大试剂。
DOI：
10.1016/0003-2697(90)90293-i
复制
发表时间：
1990
影响因子：
2.9
通讯作者：
R. Thompson
中科院分区：
文献类型：
--
作者：
S. Allenmark;M. Chelminska;R. Thompson
在水/有机两相体系中，用新试剂N-（9-吖啶基）-溴乙酰胺对多种羧酸进行了微量相转移催化酯化反应。该方法得到的酯在酸性溶液中显示出非常高的荧光强度。大的斯托克斯位移（>120 nm）意味着即使在使用大的发射带宽时也可以实现低背景。这些衍生物在使用含有0.2%磷酸的乙腈水溶液作为移动的相的反相液相色谱柱上显示出非常好的色谱行为。在最佳条件下，可获得低至10 fmol的检测限（SN = 2）。
Phase-transfer-catalyzed esterification of various carboxylic acids has been carried out on a microanalytical scale with a new reagent, N-(9-acridinyl)-bromoacetamide, in aqueous/organic two-phase systems. The procedure gives esters showing very high fluorescence intensity in acid solution. The large Stokes' shift (>120 nm) means that a low background can be achieved even when using a large emission bandwidth. These derivatives show very good chromatographic behavior on reversed-phase liquid chromatographic columns with the use of aqueous acetonitrile, containing 0.2% of phosphoric acid, as the mobile phase. Detection limits (S N = 2 ) as low as 10 fmol can be obtained under optimal conditions.