Temporally controlled targeted somatic mutagenesis in the mouse brain
Temporally controlled targeted somatic mutagenesis in the mouse brain
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DOI：
10.1046/j.0953-816x.2001.01803.x
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发表时间：
2001-12-01
影响因子：
3.4
通讯作者：
Chambon, P
中科院分区：
文献类型：
--
作者：
Weber, P;Metzger, D;Chambon, P
为了在成年小鼠神经系统中开发时空控制的体细胞诱变，我们建立了在小鼠朊病毒蛋白（PrP）启动子控制下表达他莫昔芬诱导型Cre-ERT重组酶的转基因小鼠。Cre-ERT在一个转基因系的大脑和视网膜的大部分区域中表达，而在另一个系中其表达主要限于海马和小脑。由于他莫昔芬有效地诱导Cre介导的重组在成年小鼠脑中表达Cre-ERT的各种神经元细胞类型中，PrP-Cre-ERT系应该是研究参与神经退行性疾病或再生的基因的功能以及复杂过程如行为、学习和记忆的有价值的工具。一些限制，目前可用的报告线铬介导的重组在成年小鼠中枢神经系统进行了讨论。
To develop spatio-temporally controlled somatic mutagenesis in the adult mouse nervous system, we established transgenic mice expressing the tamoxifen-inducible Cre-ERT recombinase under the control of the mouse prion protein (PrP) promoter. Cre-ERT was expressed in most regions of the brain and in the retina of one transgenic line, whereas its expression was mostly restricted to the hippocampus and the cerebellum in another line. As tamoxifen efficiently induced Cre-mediated recombination in the various neuronal cell types expressing Cre-ERT in the brain of adult mice, the PrP-Cre-ERT lines should be valuable tools to study the functions of genes involved in neurodegenerative diseases or regeneration, and in complex processes such as behaviour, learning and memory. Some limitations of presently available reporter lines for Cre-mediated recombination in adult mouse CNS are discussed.