Modeling for Gellan Gum Production by Sphingomonas paucimobilis ATCC 31461 in a Simplified Medium
Modeling for Gellan Gum Production by Sphingomonas paucimobilis ATCC 31461 in a Simplified Medium
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DOI：
10.1128/aem.72.5.3367-3374.2006
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发表时间：
2006-05
影响因子：
4.4
通讯作者：
Xia Wang;P. Xu;Yong Yuan;C. Liu;Dezhong Zhang;Zhengting Yang;Chunyu Yang;Cuiqing Ma
中科院分区：
文献类型：
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作者：
Xia Wang;P. Xu;Yong Yuan;C. Liu;Dezhong Zhang;Zhengting Yang;Chunyu Yang;Cuiqing Ma
摘要以少动鞘氨醇单胞菌ATCC 31461为研究对象，在简化培养基中进行短时间发酵，并建立了一个用于了解、控制和优化发酵过程的动力学模型。结果表明，葡萄糖是最佳碳源，最佳浓度为30 g l-1。至于发酵参数，相当大量的结冷胶产生的8小时的老文化和4%的接种在200转/分的旋转振荡器。在优化的条件下，结冷胶的最高水平（14.75克升-1）和最高的转化效率（49.17%），在30升发酵罐分批发酵。Logistic模型和Luedeking-Piret模型能够较好地描述结冷胶发酵过程，为结冷胶发酵动力学研究提供了一定的理论依据。此外，这项研究是第一次证明结冷胶生产在很大程度上是增长相关的动力学分析，在其分批发酵过程。基于模型预测，在相同的总葡萄糖浓度（30 g l − 1）下，在补料分批发酵中获得更高的结冷胶产量（17.71 g l −1）和更高的转化效率（57.12%）。
ABSTRACT Gellan gum production was carried out by Sphingomonas paucimobilis ATCC 31461 in a simplified medium with a short incubation time, and a kinetic model for understanding, controlling, and optimizing the fermentation process was proposed. The results revealed that glucose was the best carbon source and that the optimal concentration was 30 g liter−1. As for the fermenting parameters, considerably large amounts of gellan gum were yielded by an 8-h-old culture and a 4% inoculum at 200 rpm on a rotary shaker. Under the optimized conditions, the maximum level of gellan gum (14.75 g liter−1) and the highest conversion efficiency (49.17%) were obtained in a 30-liter fermentor in batch fermentation. Logistic and Luedeking-Piret models were confirmed to provide a good description of gellan gum fermentation, which gave some support for the study of gellan gum fermentation kinetics. Additionally, this study is the first demonstration that gellan gum production is largely growth associated by analysis of kinetics in its batch fermentation process. Based on model prediction, higher gellan gum production (17.71 g liter−1) and higher conversion efficiency (57.12%) were obtained in fed-batch fermentation at the same total glucose concentration (30 g liter−1).