RAPID ASSESSMENT OF DRUG SUSCEPTIBILITIES OF MYCOBACTERIUM-TUBERCULOSIS BY MEANS OF LUCIFERASE REPORTER PHAGES
RAPID ASSESSMENT OF DRUG SUSCEPTIBILITIES OF MYCOBACTERIUM-TUBERCULOSIS BY MEANS OF LUCIFERASE REPORTER PHAGES
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DOI：
10.1126/science.8484123
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发表时间：
1993-05-07
期刊：
影响因子：
56.9
通讯作者：
BLOOM, BR
中科院分区：
文献类型：
--
作者：
JACOBS, WR;BARLETTA, RG;BLOOM, BR
由于多重耐药结核分枝杆菌的出现，有效的结核病化疗需要快速评估药物敏感性。通过一种简单的方法评估药物敏感性，该方法基于被表达萤火虫荧光素酶基因的特定报告噬菌体感染的活分枝杆菌有效产生光子。光的产生取决于噬菌体感染、荧光素酶基因的表达和细胞三磷酸腺苷的水平。报告噬菌体感染强毒力结核分枝杆菌后几分钟内即可检测到信号。用抗结核药物（包括异烟肼或利福平）培养常规菌株会导致光产生消失。相比之下，耐药菌株在荧光素酶报告噬菌体感染后继续产生光信号。荧光素酶报告噬菌体可能有助于将建立结核分枝杆菌菌株抗生素敏感性所需的时间从几周缩短到几天，并加速新抗结核药物的筛选。
Effective chemotherapy of tuberculosis requires rapid assessment of drug sensitivity because of the emergence of multidrug-resistant Mycobacterium tuberculosis. Drug susceptibility was assessed by a simple method based on the efficient production of photons by viable mycobacteria infected with specific reporter phages expressing the firefly luciferase gene. Light production was dependent on phage infection, expression of the luciferase gene, and the level of cellular adenosine triphosphate. Signals could be detected within minutes after infection of virulent M. tuberculosis with reporter phages. Culture of conventional strains with antituberculosis drugs, including isoniazid or rifampicin, resulted in extinction of light production. In contrast, light signals after luciferase reporter phage infection of drug-resistant strains continued to be produced. Luciferase reporter phages may help to reduce the time required for establishing antibiotic sensitivity of M. tuberculosis strains from weeks to days and to accelerate screening for new antituberculosis drugs.