Longitudinal multi-locus molecular characterisation of sporadic Australian human clinical cases of cryptosporidiosis from 2005 to 2008
Longitudinal multi-locus molecular characterisation of sporadic Australian human clinical cases of cryptosporidiosis from 2005 to 2008
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DOI：
10.1016/j.exppara.2010.02.017
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发表时间：
2010-08-01
影响因子：
2.1
通讯作者：
Ryan, Una
中科院分区：
文献类型：
--
作者：
Ng, Josephine;MacKenzie, Brian;Ryan, Una
隐孢子虫是一种胃肠道寄生虫，被认为是发展中国家和工业化国家非病毒性腹泻的重要原因。在本研究中，对2005年至2008年澳大利亚人胃肠道症状的248份粪便标本进行了纵向分析。18 S rRNA基因位点和60 kDa糖蛋白（gp 60）基因位点的序列分析显示，195例（78.6%）的病例是由于感染隐孢子虫人，49例（19.8%）的隐孢子虫parvum和4例（1.6%）的隐孢子虫隐孢子虫。共鉴定出8个gp 60亚型家族; 5个人型衣原体亚型家族（Ib. Id、le、If和IG）和两个C. parvum亚型家族（IIa和IId）。Id亚型家族是最常见的C.在45.7%的分离株中鉴定出人亚型家族，其次是IG亚型家族（30.3%）和Ib亚型家族（20%）。最常见的C parvum亚型是IIaA 18 G3 R1，在65.3%的分离株中鉴定。在一个分离株中发现了更罕见的人畜共患眼睑A15 G1亚型。统计学分析显示Id亚型与腹痛相关散发性隐孢子虫病5岁及以下儿童感染Id亚型的可能性分别是散发性隐孢子虫病的1.91倍和1.88倍（RR 1.91; 95% CI，1.7-2.89; p < 0.05）和IG（RR 1.88; 95% CI，1.10-3.24; p < 0.05）。还在可变CP 47和MSC 6 -7基因座上分析了分离株的一个子集。这项研究的结果表明，隐孢子虫的人传人在西澳大利亚人隐孢子虫病的流行病学中起着重要作用。(C)2010年爱思唯尔公司All rights reserved.
Cryptosporidium is a gastrointestinal parasite that is recognised as a significant cause of non-viral diarrhea in both developing and industrialised countries. In the present study, a longitudinal analysis of 248 faecal specimens from Australian humans with gastrointestinal symptoms from 2005 to 2008 was conducted. Sequence analysis of the 18S rRNA gene locus and the 60 kDa glycoprotein (gp60) gene locus revealed that 195 (78.6%) of the cases were due to infection with Cryptosporidium hominis, 49(19.8%) with Cryptosporidium parvum and four (1.6%) with Cryptosporidium meleagridis. A total of eight gp60 subtype families were identified; five C hominis subtype families (Ib. Id, le, If and Ig), and two C. parvum subtype families (IIa and IId). The Id subtype family was the most common C. hominis subtype family identified in 45.7% of isolates, followed by the Ig subtype family (30.3%) and the Ib subtype family (20%). The most common C parvum subtype was IIaA18G3R1, identified in 65.3% of isolates. The more rare zoonotic lid A15G1 subtype was identified in one isolate. Statistical analysis showed that the Id subtype was associated with abdominal pain (p < 0.05) and that in sporadic cryptosporidiosis, children aged 5 and below were 1.91 times and 1.88 times more likely to be infected with subtype Id (RR 1.91; 95% CI, 1.7-2.89; p < 0.05) and Ig (RR 1.88; 95% CI, 1.10-3.24; p < 0.05) compared to children aged 5 and above. A subset of isolates were also analysed at the variable CP47 and MSC6-7 gene loci. Findings from this study suggest that anthroponotic transmission of Cryptosporidium plays a major role in the epidemiology of cryptosporidiosis in Western Australian humans. (C) 2010 Elsevier Inc. All rights reserved.