Syrinx 2A: an improved lambda phage vector designed for screening DNA libraries by recombination in vivo.
Syrinx 2A: an improved lambda phage vector designed for screening DNA libraries by recombination in vivo.
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Syrinx 2A：一种改进的 lambda 噬菌体载体，设计用于通过体内重组筛选 DNA 文库。
DOI：
10.1073/pnas.84.13.4379
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发表时间：
1987
影响因子：
11.1
通讯作者：
Huang,HV
中科院分区：
文献类型：
--
作者：
Lutz,CT;Hollifield,WC;Seed,B;Davie,JM;Huang,HV
设计了Syrinx 2A噬菌体和pi AN 13质粒，用于通过体内同源重组筛选DNA文库。Syrinx 2A携带多个克隆位点和一个最近鉴定的λ基因，rap（与质粒的重组熟练），需要有效的噬菌体质粒重组。我们描述了一种快速，可靠，技术上简单的方法来筛选Syrinx 2A库，扩大所产生的噬菌体质粒共整合，并在短短2天内亚克隆质粒。分离筛选允许选择性地分离密切相关的多基因家族的一个特定成员。
The Syrinx 2A phage and pi AN13 plasmid were designed for screening of DNA libraries by homologous recombination in vivo. Syrinx 2A carries multiple cloning sites and a recently identified lambda gene, rap (recombination adept with plasmid), required for efficient phage-plasmid recombination. We describe a rapid, reliable, and technically easy method to screen Syrinx 2A libraries, expand the resulting phage-plasmid cointegrates, and subclone plasmid in as little as 2 days. Recombination screening allows one specific member of a closely related multigene family to be isolated selectively.