Sexually dimorphic gene expression in mouse brain precedes gonadal differentiation
Sexually dimorphic gene expression in mouse brain precedes gonadal differentiation
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DOI：
10.1016/s0169-328x(03)00339-5
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发表时间：
2003-10-21
期刊：
影响因子：
--
通讯作者：
Vilain, E
中科院分区：
文献类型：
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作者：
Dewing, P;Shi, T;Vilain, E
大脑性别分化和行为的经典观点是，性腺类固醇激素直接促进神经和行为发育的性别差异。特别是，睾酮及其代谢物的作用会诱导大脑发育的男性模式，同时抑制女性神经和行为模式的分化。然而，最近的证据表明，性腺激素可能不仅仅导致男性和女性之间大脑发育和行为的性别差异。在这里，我们研究了另一种假设，即基因通过直接诱导神经发育的性别二态性模式，可以影响男性和女性大脑之间的性别差异。使用微阵列和 RT-PCR，我们检测到了 50 多个性别表达差异的候选基因，并确认了至少 7 个小鼠基因，这些基因在性交后 10.5 天（dpc）阶段（在任何性腺激素影响之前）雄性和雌性小鼠的大脑发育中表现出差异表达。在性腺形成之前，男性和女性大脑之间差异表达的基因的鉴定表明，遗传因素可能在影响大脑性别分化方面发挥作用。 (C) 2003 Elsevier B.V. 保留所有权利。
The classic view of brain sexual differentiation and behavior is that gonadal steroid hormones act directly to promote sex differences in neural and behavioral development. In particular, the actions of testosterone and its metabolites induce a masculine pattern of brain development, while inhibiting feminine neural and behavioral patterns of differentiation. However, recent evidence indicates that gonadal hormones may not solely be responsible for sex differences in brain development and behavior between males and females. Here we examine an alternative hypothesis that genes, by directly inducing sexually dimorphic patterns of neural development, can influence the sexual differences between male and female brains. Using microarrays and RT-PCR, we have detected over 50 candidate genes for differential sex expression, and confirmed at least seven murine genes which show differential expression between the developing brains of male and female mice at stage 10.5 days post coitum (dpc), before any gonadal hormone influence. The identification of genes differentially expressed between male and female brains prior to gonadal formation suggests that genetic factors may have roles in influencing brain sexual differentiation. (C) 2003 Elsevier B.V. All rights reserved.