Renal nerves modulate renin gene expression in the developing rat kidney with ureteral obstruction.
Renal nerves modulate renin gene expression in the developing rat kidney with ureteral obstruction.
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DOI：
10.1172/jci115083
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发表时间：
1991-03
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影响因子：
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通讯作者：
Samir S. El-Dahr;R. A. Gomez;Mark S. Gray;M. J. Peach;R. M. Carey;Robert L. Chevalier;M. Garmey-
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文献类型：
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作者：
Samir S. El-Dahr;R. A. Gomez;Mark S. Gray;M. J. Peach;R. M. Carey;Robert L. Chevalier;M. Garmey-
新生大鼠慢性单侧输尿管梗阻（UUO）激活梗阻肾中的肾素基因表达，并增加肾素沿沿着传入肾小球小动脉在双肾中的分布。为了研究肾神经在这种反应中的作用，2日龄Sprague-Dawley大鼠进行UUO或假手术。通过注射乙啶进行化学交感神经切除术，而对照组接受生理盐水载体。在4-5周时，用免疫细胞化学法测定肾脏肾素分布，用北方印迹杂交法测定肾素mRNA水平。与生理盐水治疗的UUO大鼠相比，接受乙啶治疗的UUO大鼠的肾脏中的肾素仍然定位于肾小球区域（P <0.05）。此外，在接受乙啶的大鼠阻塞肾脏中，肾素mRNA水平比接受生理盐水的大鼠低8倍。UUO大鼠的其他组进行单侧机械肾去神经：阻塞肾脏中的肾素基因表达被抑制同侧，但不是对侧肾去神经。这些结果表明，无论是化学或机械去神经抑制的同侧UUO新生儿肾脏的肾素基因表达的增加。我们的结论是肾交感神经调节肾素基因表达的发展中的肾脏慢性UUO。
Chronic unilateral ureteral obstruction (UUO) in newborn rats activates renin gene expression in the obstructed kidney, and increases renin distribution along afferent glomerular arterioles in both kidneys. To investigate the role of the renal nerves in this response, 2-d-old Sprague-Dawley rats were subjected to UUO or sham operation. Chemical sympathectomy was performed by injection of guanethidine, whereas, control groups received saline vehicle. At 4-5 wk, renal renin distribution was determined by immunocytochemistry, and renin mRNA levels were determined by Northern blot hybridization. Compared to the saline-treated rats with UUO, renin remained localized to the juxtaglomerular region in both kidneys of rats with UUO receiving guanethidine (P less than 0.05). Moreover, renin mRNA levels were eightfold lower in obstructed kidneys of rats receiving guanethidine than in those receiving saline. Additional groups of rats with UUO were subjected to unilateral mechanical renal denervation: renin gene expression in the obstructed kidney was suppressed by ipsilateral but not by contralateral renal denervation. These findings indicate that either chemical or mechanical denervation suppressed the increase in renin gene expression of the neonatal kidney with ipsilateral UUO. We conclude that the renal sympathetic nerves modulate renin gene expression in the developing kidney with chronic UUO.