EFFECTS OF BASIC FIBROBLAST GROWTH-FACTOR AND HEPARIN ON FOLLICLE-STIMULATING HORMONE-INDUCED STEROIDOGENESIS BY BOVINE GRANULOSA-CELLS
EFFECTS OF BASIC FIBROBLAST GROWTH-FACTOR AND HEPARIN ON FOLLICLE-STIMULATING HORMONE-INDUCED STEROIDOGENESIS BY BOVINE GRANULOSA-CELLS
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DOI：
10.2527/1994.72102696x
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发表时间：
1994-10-01
影响因子：
3.3
通讯作者：
SPICER, LJ
中科院分区：
文献类型：
--
作者：
VERNON, RK;SPICER, LJ
本研究的目的是确定碱性成纤维细胞生长因子（bFGF）和肝素，一种主要的bFGF结合蛋白，对牛颗粒细胞雌二醇和孕酮的生产的影响和相互作用。从牛的小卵泡（1 ~ 5 mm）中收集细胞，在含10%胎牛血清的培养基中培养2d，然后在无血清培养基中用bFGF和（或）肝素处理1d。用0.1 - 10 μ g/mL肝素处理1天没有影响（P > 0.05），而100 μ g/mL肝素抑制（P <0.05）FSH诱导的牛颗粒细胞雌二醇产生。用0.1 ~ 1.0 ng/mL bFGF处理1d，并以人血清白蛋白作为载体蛋白，抑制FSH诱导的牛颗粒细胞雌二醇的产生（P <0.05），在1.0 ng/mL时检测到最大的抑制作用;与10 μ g/mL肝素共培养不影响这些作用。相比之下，0.1 ng/mL无载体bFGF对FSH诱导的雌二醇产生没有影响（P > 0.05），而1.0 - 100 ng/mL无载体bFGF抑制FSH诱导的雌二醇产生（P <0.05），在100 ng/mL时检测到最大抑制。肝素（10 μ g/mL）的存在下，颗粒细胞的雌二醇和孕酮的生产受到无载体bFGF的抑制有不同的影响。这些结果表明，碱性成纤维细胞生长因子可能在牛卵泡发育过程中FSH调节的颗粒细胞类固醇生成中发挥重要作用。
The objectives of the present study were to determine the effect of and interaction between basic fibroblast growth factor (bFGF) and heparin, a major bFGF-binding protein, on bovine granulosa cell estradiol and progesterone production. Cells from small (1 to 5 mm) follicles were collected from cattle, cultured for 2 d in medium containing 10% fetal calf serum, and then treated for 1 d with bFGF and(or) heparin in serum-free medium. Treatment with .1 to 10 mu g/mL of heparin for 1 d had no effect (P > .05), whereas 100 mu g/mL of heparin inhibited (P < .05) FSH-induced estradiol production by bovine granulosa cells. Treatment with .1 to 1.0 ngl mt of bFGF for l d, supplied with human serum albumin as a carrier protein, inhibited(P < .05) FSH-induced estradiol production by bovine granulosa cells, with the greatest inhibition detected at 1.0 ng/mL; coculture with 10 mu g/mL of heparin did not influence these effects. In comparison,.1 ng/mL of bFGF supplied carrier-free had no effect (P > .05) on FSH-induced estradiol production, whereas 1.0 to 100 ng/ml of carrier-free bFGF inhibited (P < .05) FSH-induced estradiol production, with the greatest inhibition detected at 100 ng/mL. The presence of heparin (10 mu g/mL) had variable effects on granulosa cell estradiol and progesterone production inhibited by carrier-free bFGF. These results indicate that bFGF may play a significant role in FSH-modulated granulosa cell steroidogenesis during follicular development in cattle.