Factors affecting the first cleavage interval and effects of parental generation on tetraploid production in rainbow trout (Oncorhynchus mykiss)
Factors affecting the first cleavage interval and effects of parental generation on tetraploid production in rainbow trout (Oncorhynchus mykiss)
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DOI：
10.1016/j.aquaculture.2012.03.017
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发表时间：
2012-05
期刊：
影响因子：
4.5
通讯作者：
G. M. Weber;Mark A. Hostuttler
中科院分区：
文献类型：
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作者：
G. M. Weber;Mark A. Hostuttler
通过在授精和第一次卵裂之间的特定时间点或第一次卵裂间隔（FCI）施加压力冲击，在虹鳟鱼中诱导四倍体。以前的研究表明，个体和鱼类种群之间FCI的变化阻止了对所有鳟鱼的单一时间点的识别。在这项研究中，我们证实了施加压力的最佳时间是FCI的65±5%。此外，我们发现，从一个共同的环境中的鱼的FCI的变化可以在允许一个单一的时间点，以建立该组鱼的限度内，如果考虑到排卵后老化的卵子。老化的卵子，无论是在体内还是在体外，增加FCI的程度是一个关注四倍体诱导。FCI在排卵后7 d比1d延长约12 min，10- 14 d延长约30 min。一组鱼的FCI是一致的整个产卵季节。第一代和第二代四倍体雄性的后代的存活率和脊髓异常的频率相似，但与第一代雌性相比，第二代四倍体雌性的存活率增加了一倍，异常减少了约90%。四倍体杂交后代经胚胎细胞流式细胞仪检测均为四倍体。总之，注意卵老化和使用第二代雌性四倍体允许四倍体虹鳟亲鱼的有效生产。
Tetraploidy is induced in rainbow trout by applying a pressure shock at a specific time point between insemination and first cleavage, or the first cleavage interval (FCI). Previous studies suggested that variation in the FCI among individuals and populations of fish prevents the identification of a single time point that can be used for all trout. In this study we confirmed the optimal time to apply pressure is 65±5% of the FCI. In addition, we found that variation in FCI of fish from a common environment can be within limits that allow a single time point to be established for that group of fish, if ova post ovulatory aging is taken into account. Aging of ova, either in vivo or in vitro, increased FCI to a degree that is a concern for tetraploid induction. The FCI was about 12min longer at 7days post ovulation, and 30min at 10–14days, than at 1day. The FCI for a group of fish was consistent throughout the spawning season. Survival to hatching and frequency of spinal abnormalities were similar for progeny of first and second generation tetraploid males, but survival was doubled and abnormalities reduced by approximately 90% in second generation tetraploid females compared with first generation females. All progeny of tetraploid by tetraploid crosses were determined to be tetraploids based on flow cytometry of embryonic cells. In summary, attention to ova aging and use of second generation female tetraploids allows efficient production of a tetraploid rainbow trout broodstock.