Flavonoids such as luteolin, fisetin and apigenin are inhibitors of interleukin-4 and interleukin-13 production by activated human basophils
Flavonoids such as luteolin, fisetin and apigenin are inhibitors of interleukin-4 and interleukin-13 production by activated human basophils
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DOI：
10.1159/000078498
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发表时间：
2004-01-01
影响因子：
2.8
通讯作者：
Tanaka, T
中科院分区：
文献类型：
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作者：
Hirano, T;Higa, S;Tanaka, T
背景资料：我们以前已经表明，非瑟酮，黄酮醇，抑制过敏原或抗IgE抗体刺激的嗜碱性粒细胞的IL-4和IL-13的合成。这次，我们研究了其他类黄酮对嗜碱性粒细胞产生IL-4和IL-13的抑制作用，并试图确定与抑制作用相关的类黄酮的基本结构。我们还研究了类黄酮是否抑制嗜碱性粒细胞的白三烯C4合成和T细胞对抗CD 3抗体的IL-4合成。研究方法：在不同浓度的18种黄酮类化合物和黄酮醇类化合物的存在下，用抗IgE抗体单独或抗IgE抗体加IL-3刺激高度纯化的外周嗜碱性粒细胞12小时。然后测量上清液中的IL-4和IL-13浓度。在类黄酮存在下刺激嗜碱性粒细胞1小时后，还测量了白三烯C4的合成。关于类黄酮对T细胞合成IL-4的抑制活性，将外周血单核细胞与类黄酮在抗CD 3抗体结合的板中培养2天。结果如下：木犀草素，非瑟酮和芹菜素被认为是最强的抑制剂的IL-4和IL-13的生产嗜碱性粒细胞，但不影响白三烯C4的合成。在较高浓度下，这些黄酮类化合物抑制T细胞产生IL-4。基于抑制活性的层次结构，确定了嗜碱性粒细胞抑制IL-4的基本结构。结论：由于类黄酮对IL-4和IL-13合成的抑制活性，可以预期，类黄酮的摄入量（取决于数量和质量）可以改善过敏症状或预防过敏性疾病的发作。版权所有（C）2004 S. Karger AG，巴塞尔。
Background: We have previously shown that fisetin, a flavonol, inhibits IL-4 and IL-13 synthesis by allergen- or anti-IgE-antibody-stimulated basophils. This time, we investigated the inhibition of IL-4 and IL-13 production by basophils by other flavonoids and attempted to determine the fundamental structure of flavonoids related to inhibition. We additionally investigated whether flavonoids suppress leukotriene C4 synthesis by basophils and IL-4 synthesis by T cells in response to anti-CD3 antibody. Methods: Highly purified peripheral basophils were stimulated for 12 h with anti-IgE antibody alone or anti-IgE antibody plus IL-3 in the presence of various concentrations of 18 different kinds of flavones and flavonols. IL-4 and IL-13 concentrations in the supernatants were then measured. Leukotriene C4 synthesis was also measured after basophils were stimulated for 1 h in the presence of flavonoids. Regarding the inhibitory activity of flavonoids on IL-4 synthesis by T cells, peripheral blood mononuclear cells were cultured with flavonoids in anti-CD3-antibody-bound plates for 2 days. Results: Luteolin, fisetin and apigenin were found to be the strongest inhibitors of both IL-4 and IL-13 production by basophils but did not affect leukotriene C4 synthesis. At higher concentrations, these flavonoids suppressed IL-4 production by T cells. Based on a hierarchy of inhibitory activity, the basic structure for IL-4 inhibition by basophils was determined. Conclusions: Due to the inhibitory activity of flavonoids on IL-4 and IL-13 synthesis, it can be expected that the intake of flavonoids, depending on the quantity and quality, may ameliorate allergic symptoms or prevent the onset of allergic diseases. Copyright (C) 2004 S. Karger AG, Basel.