Recreating the hematon: microfabrication of artificial haematopoietic stem cell microniches in vitro using dielectrophoresis
Recreating the hematon: microfabrication of artificial haematopoietic stem cell microniches in vitro using dielectrophoresis
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DOI：
10.1007/s10544-008-9219-y
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发表时间：
2009-02
影响因子：
2.8
通讯作者：
G. Markx;L. Carney;M. Littlefair;Anil Sebastian;A. Buckle
中科院分区：
文献类型：
--
作者：
G. Markx;L. Carney;M. Littlefair;Anil Sebastian;A. Buckle
血液是一种三维的细胞聚集体，能够产生所有的血型。为了能够做到这一点，它必须能够在细胞聚集体内创造一个微环境，使造血干细胞能够维持，更新和分化。第一步是在体外通过介电泳产生高度为50-100 μm的半球形细胞聚集体来产生人工造血干细胞微环境，所述半球形细胞聚集体具有与推定的血细胞相似的确定的内部结构。结果表明，在介电泳操作后，细胞保持活力和活性。聚集体内的细胞彼此直接接触，潜在地允许细胞构建体内的直接细胞-细胞通信。探索了一些细胞固定化方法，用于在其形成后进一步稳定细胞聚集体的3-D组织。证明了将可追踪的单个细胞引入到人工显微镜中。
The hematon is a three-dimensional aggregate of cells which is able to produce all blood types. To be able to do this, it must be able to create within the cell aggregate a microenvironment which enables haematopoietic stem cell maintenance, renewal and differentiation. A first step was taken towards the creation of artificial hematopoietic stem cell microniches in vitro by the creation with dielectrophoresis of hemispherical cell aggregates of a height of 50–100 μm with a defined internal architecture similar to that of a putative hematon. It is shown that, after their dielectrophoretic manipulation, the cells remain viable and active. Cells within the aggregate are in direct contact with each other, potentially allowing direct cell–cell communication within the cell construct. Some cell immobilisation methods are explored for further stabilising the 3-D organisation of the cell aggregate after its formation. The introduction of traceable individual cells into the artificial microniche is demonstrated.