Early growth response 2 (Egr2) plays opposing roles in committing C3H10T1/2 stem cells to adipocytes and smooth muscle-like cells.
Early growth response 2 (Egr2) plays opposing roles in committing C3H10T1/2 stem cells to adipocytes and smooth muscle-like cells.
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DOI：
10.1016/j.biocel.2013.06.003
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发表时间：
2013-08
期刊：
影响因子：
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通讯作者：
Shan‐Shan Wang;Haiyan Huang;Su-Zhen Chen;Xi Li;Yang Liu;Wenting Zhang;Q. Tang
中科院分区：
文献类型：
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作者：
Shan‐Shan Wang;Haiyan Huang;Su-Zhen Chen;Xi Li;Yang Liu;Wenting Zhang;Q. Tang
早期生长反应2 （Early growth response 2, Egr2）是一种锌指转录因子，在细胞分化、增殖和凋亡等多种生理过程中起着重要的调节作用。本研究表明，在C3H10T1/2干细胞向脂肪细胞谱系转变的过程中，骨形态发生蛋白（BMP）信号通路下调了Egr2。过表达Egr2完全阻止了bmp4诱导的C3H10T1/2干细胞的脂肪细胞系承诺，同时刺激了早期平滑肌样分化。我们还证明了egr2诱导的早期平滑肌样分化是不依赖于转化生长因子β1的。Egr2可以激活早期平滑肌细胞特异性基因平滑肌蛋白22α和钙钙蛋白1的转录。总之，结果表明Egr2在抑制脂肪细胞谱系承诺和促进早期平滑肌样细胞分化中的新作用。
Early growth response 2 (Egr2) is a zinc-finger transcription factor that acts as an important modulator of a variety of physiological processes, such as cell differentiation, proliferation and apoptosis. Here we showed that Egr2 was downregulated by bone morphogenetic protein (BMP) signaling pathways during the commitment of C3H10T1/2 stem cells to adipocyte lineage. Overexpression of Egr2 completely prevented BMP4-induced adipocyte lineage commitment of C3H10T1/2 stem cells, while simultaneously stimulating early smooth muscle-like differentiation. We also demonstrated that Egr2-induced early smooth muscle-like differentiation is transforming growth factor β1-independent. Egr2 can activate the transcription of early smooth muscle cell specific genes smooth muscle protein 22α and calponin 1. Together, the results indicated a novel role for Egr2 in repressing adipocyte lineage commitment and promoting early smooth muscle-like cell differentiation.