MRC OX-19 - A MONOCLONAL-ANTIBODY THAT LABELS RAT LYMPHOCYTES-T AND AUGMENTS INVITRO PROLIFERATIVE RESPONSES
MRC OX-19 - A MONOCLONAL-ANTIBODY THAT LABELS RAT LYMPHOCYTES-T AND AUGMENTS INVITRO PROLIFERATIVE RESPONSES
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DOI：
10.1002/eji.1830140311
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发表时间：
1984-01-01
影响因子：
5.4
通讯作者：
GREEN, JR
中科院分区：
文献类型：
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作者：
DALLMAN, MJ;THOMAS, ML;GREEN, JR
制备了一种小鼠单克隆抗体，命名为 MRC OX-19，它几乎可以与所有大鼠胸腺细胞结合。 T 淋巴细胞和最多 2% B 淋巴细胞。组织切片的免疫过氧化物酶研究显示与肠、胸腺、脾脏和淋巴结中的非淋巴细胞没有反应性。通过胸腺细胞的代谢和细胞表面标记，然后进行免疫沉淀和十二烷基硫酸钠凝胶电泳来鉴定 MRC OX-19 抗体识别的抗原。它是一种 MW = 69,000 的表面糖蛋白。当包含在 T 细胞功能的体外测定中时，MRC OX-19 增加了同种异体脾细胞或凝集素植物血凝素和刀豆球蛋白 A 刺激的增殖。该抗体本身不具有促有丝分裂作用，其刺激作用可能与白细胞介素 2 产量的增加相关。 MRC OX-19 可能相当于小鼠 Ly-1 抗原和人 T1 抗原。
A mouse monoclonal antibody, designated MRC OX-19, was prepared that binds to virtually all rat thymocytes. T lymphocytes and a maximum of 2% B lymphocytes. Immunoperoxidase studies on tissue sections showed no reactivity with non-lymphoid cells in intestine, thymus, spleen and lymph node. The antigen recognized by MRC OX-19 antibody was identified by metabolic and cell surface labeling of thymocytes followed by immunoprecipitation and sodium dodecyl sulfate gel electrophoresis. It is a surface glycoprotein of MW = 69,000. When included in in vitro assays for T cell functions, MRC OX-19 increased proliferation stimulated by allogenic spleen cells or the lectins phytohemagglutinin and and concanavalin A. The antibody itself was not mitogenic and its stimulatory effect could be correlated with an increase in interleukin 2 production. MRC OX-19 could be the equivalent of mouse Ly-1 antigen and human T1 antigen.