ASSOCIATION OF VIRAL REVERSE TRANSCRIPTASE WITH AN ENZYME DEGRADING RNA MOIETY OF RNA-DNA HYBRIDS
ASSOCIATION OF VIRAL REVERSE TRANSCRIPTASE WITH AN ENZYME DEGRADING RNA MOIETY OF RNA-DNA HYBRIDS
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DOI：
10.1038/newbio234240a0
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发表时间：
1971-01-01
期刊：
影响因子：
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通讯作者：
HAUSEN, P
中科院分区：
文献类型：
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作者：
MOLLING, K;BOLOGNESI, DP;HAUSEN, P
在 RNA 肿瘤病毒的复制过程中，病毒 RNA 中包含的遗传信息似乎被转移到 DNA1,2 中。对病毒颗粒中酶活性的研究表明，这种转移是由 RNA 依赖性 DNA 聚合酶介导的 3,4。 RNA-DNA 杂交体已被证明作为该反应中的中间体出现，并且单链 DNA 作为早期反应产物 6 产生，然后复制以产生双链 DNA 产物 6-8。然而，单链 DNA 从 RNA 模板中置换出来的机制尚不清楚。
DURING replication of RNA tumour viruses, the genetic information contained in the viral RNA seems to be transferred to DNA1,2. Studies on the enzymatic activities present in the virus particles suggest that this transfer is mediated by an RNA dependent DNA polymerase3,4. RNA-DNA hybrids have been demonstrated to occur as intermediates in this reaction5, and single stranded DNA is generated as an early reaction product6, which is then replicated to give a double stranded DNA product6–8. The mechanism by which the single stranded DNA is displaced from the RNA template is, however, not known.