Nitric oxide synthesis inhibition suppresses implantation and decreases cGMP concentration and protein peroxidation
Nitric oxide synthesis inhibition suppresses implantation and decreases cGMP concentration and protein peroxidation
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DOI：
10.1016/s0024-3205(99)00491-9
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发表时间：
1999-10-15
期刊：
影响因子：
6.1
通讯作者：
Hicks-Gómez, JJ
中科院分区：
文献类型：
--
作者：
Durán-Reyes, G;Gómez-Meléndez, MD;Hicks-Gómez, JJ
在大鼠植入部位（IS）和非植入部位（NIS）评估Nw-硝基-L-精氨酸对胚胎植入和cGMP羰基浓度的影响。管腔内施用 25 μg (2.3 mM) Nw-硝基-L-精氨酸可抑制 34.7% 的着床率和胚胎存活率 (100%)，同时还降低了该位点的 cGMP 浓度（对照为 1664.2 +/- 333.8 pmoles/mg 蛋白质，治疗组为 1321 +/- 384.3 pmoles/mg 蛋白质）以及 NIS （1203.7 +/- 200 至 780.2 +/- 168.5）。用Nw-硝基-L-精氨酸处理的植入位点处的羰基浓度显着低于对照（分别为0.062+/-0.012纳摩尔/毫克蛋白质和0.45+/-0.1）。尽管如此，NIS 并没有显着差异（0.12 +/- 0.04 和 0.15 +/- 0.05）。我们的结果表明，一氧化氮 (NO) 依赖性系统与 cGMP 和蛋白质过氧化产物的形成平行，在囊胚植入部位非常重要，以便子宫内膜获得足够的容受性所需的特性。
The effect of Nw-nitro-L-arginine on embryonic implantation and cGMP carbonyl group concentration was assessed at the rat implantation site (IS) and non-implamtation site (NIS). The intraluminal administration of 25 mu g (2.3 mM) of Nw-nitro-L-arginine inhibited implantation in 34.7% and embryo survival (100%), while in addition, decreasing cGMP concentration both at the site (1664.2 +/- 333.8 pmoles/mg of protein for the control and 1321 +/- 384.3 for those treated), as well as at the NIS (1203.7 +/- 200 to 780.2 +/- 168.5). Carbonyl group concentration was considerably less at the implantation site treated with Nw-nitro-L-arginine than in the control (0.062 +/- 0.012 nmoles/mg of protein and 0.45 +/- 0.1, respectively). Nonetheless, the NIS was not significantly different (0.12 +/- 0.04 and 0.15 +/- 0.05). Our results show that a nitric oxide (NO) dependent system parallel to the formation of cGMP and protein peroxidation products is important at the blastocyst implantation site in order for the endometrium to acquire the necessary properties for an adequate receptivity.