Sensitive detection of ascorbic acid and alkaline phosphatase activity by double-channel photoelectrochemical detection design based on g-C 3 N 4 /TiO 2 nanotubes hybrid film
Sensitive detection of ascorbic acid and alkaline phosphatase activity by double-channel photoelectrochemical detection design based on g-C 3 N 4 /TiO 2 nanotubes hybrid film
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DOI：
10.1016/j.snb.2016.02.059
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发表时间：
2016-07
影响因子：
8.4
通讯作者：
Qi Kang;W. Xuxiang;Xiaolong Ma;Lingqian Kong;Ping Zhang;Dazhong Shen
中科院分区：
文献类型：
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作者：
Qi Kang;W. Xuxiang;Xiaolong Ma;Lingqian Kong;Ping Zhang;Dazhong Shen
本工作开发了一种简单的双通道检测方法，以提高光电化学(PEC)传感器的稳定性和可靠性。其工作原理是基于测量和参考PEC单元之间的差分信号。与现有的单通道PEC模式相比，这种测量方法的优势在于，由于系统的区分性质，减小了激励源强度漂移的影响，从而产生更好的信噪比，从而提高了测量灵敏度。此外，差动测量方案减少了矩阵效应的干扰。基于g-C3N4/TiO2纳米管阵列杂化膜的差示PEC模式，实现了抗坏血酸和碱性磷酸酶的灵敏检测。该传感器对抗坏血酸的浓度变化范围为1~10μM，检测下限为0.3nM。血清中碱性磷酸酶活力测定的定量范围为0.3mU/L-1，检出限为0.1mU/L。
This work developed a simple double-channel detection approach to improve the stability and credibility in photoelectrochemical (PEC) sensors. The working principle is based on differential signal between the measuring and reference PEC cells. This measurement approach offers the advantage over established single-channel PEC mode in that the effect of the drift in the intensity of excitation source is diminished owing to the differentiating nature of the system, leading to better signal-to-noise ratio and hence higher measurement sensitivity. Additionally, the differential measurement scheme reduced the interference from matrix effect. Sensitive detection of ascorbic acid and alkaline phosphatase activity by the differential PEC mode based on g-C3N4/TiO2nanotube array hybrid film was demonstrated. The as-prepared PEC sensor offeres signal-enhancement response to ascorbic acid in a concentration variation from 1 nM to 10 μM with a detection limit of 0.3 nM. The determination the activity of alkaline phosphatase in serum samples enabled a quantification range of 0.3 mU/L–1 U/L with a detection limit of 0.1 mU/L.