Changes in connexin 43 protein expression in human endometrial carcinoma
Changes in connexin 43 protein expression in human endometrial carcinoma
复制标题
DOI：
10.1006/exmp.1999.2286
复制
发表时间：
1999-12-01
影响因子：
3.6
通讯作者：
Kaufman, DG
中科院分区：
文献类型：
--
作者：
Schlemmer, SR;Novotny, DB;Kaufman, DG
应用免疫组化和Western blot分析子宫内膜上皮来源的细胞系，研究连接蛋白43的表达。检查连接蛋白，因为其表达和功能的降低与癌发生相关。选择细胞系以代表从FEEC（胎儿子宫内膜上皮细胞;用SV 40大T抗原转化）到HEC-1A（1A期子宫内膜癌）到RL-95-2（2级子宫内膜癌）的子宫内膜癌进展的递增等级。使用连接蛋白43多克隆抗体进行免疫印迹和免疫荧光的平行研究表明，连接蛋白43的表达水平为正常子宫内膜间质细胞= FEEC > HEC-1A > RL-95-2。因此，我们应用免疫荧光法分析石蜡包埋的子宫切片，子宫切除术标本的正常子宫内膜患者和诊断为1级，2级和3级子宫内膜癌的患者。使用每个类别中的五个不同病例，我们发现连接蛋白43表达与肿瘤分级之间呈负相关。我们的数据表明，连接蛋白43的表达可能是有用的子宫内膜癌的进展作为一个连续的标志物。(C)北京：科学出版社.
The expression of connexin 43 was studied using immunohistochemical and Western blot analyses on cell lines of endometrial epithelial origin. Connexin proteins were examined because decreases in their expression and function have been correlated with carcinogenesis. The cell lines were chosen to represent increasing grades of endometrial cancer progression starting from FEEC (fetal endometrial epithelial cells; transformed with SV40 large T antigen) to HEC-1A (stage 1A endometrial carcinoma) to RL-95-2 (grade 2 endometrial carcinoma). Parallel studies using connexin 43 polyclonal antibodies for both Western blots and immunofluorescence showed that the levels of connexin 43 expression were normal endometrial stromal cells = FEEC > HEC-1A > RL-95-2. Consequently, we applied the immunofluorescence assay to analyze paraffin-embedded uterine sections from hysterectomy specimens of patients with normal endometrium and from patients diagnosed with grade 1, 2, and 3 endometrial cancer. Using five different cases from each category, we found an inverse correlation between connexin 43 expression and tumor grade. Our data indicate that connexin 43 expression may be useful as an adjunctive marker of progression for endometrial carcinoma. (C) 1999 Academic Press.