Inducible expression of calcyclin, a gene with strong homology to S-100 protein, during neuroblastoma cell differentiation and its prevalent expression in Schwann-like cell lines.
Inducible expression of calcyclin, a gene with strong homology to S-100 protein, during neuroblastoma cell differentiation and its prevalent expression in Schwann-like cell lines.
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发表时间：
1991-03
期刊：
影响因子：
11.2
通讯作者：
Gian Paolo Tonini;A. Casalaro;Andrea Cara;D. D. Martino-D.
中科院分区：
文献类型：
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作者：
Gian Paolo Tonini;A. Casalaro;Andrea Cara;D. D. Martino-D.
在不同的神经母细胞瘤细胞系和视黄酸诱导的神经元分化过程中评估钙周期素基因的表达。钙周期素基因表达更频繁地检测到上皮型或雪旺样细胞，而不是在神经母细胞。该结果表明G1细胞分数增加，这可以解释通常在这些细胞中观察到的有限生长潜力。LAN-5细胞（神经元型）分化实验表明，在视黄酸处理4天后可检测到钙周期蛋白基因，其诱导G1期积累（如通过细胞荧光分析检测到的）和细胞生长停滞。另外，无论是用0.5%胎牛血清培养基阻断细胞增殖还是在细胞停滞后加入15%新鲜胎牛血清都不能诱导钙周期素的表达。细胞分化过程中钙周期素mRNA水平的增加表明钙周期素基因表达与神经元分化有关。钙周期蛋白基因的这种二价作用通常在细胞周期的G1期表达，但也在视黄酸诱导的神经母细胞瘤细胞分化过程中表达，表明（至少在神经母细胞瘤细胞中）该基因受到复杂的转录调控。
Calcyclin gene expression was evaluated in different neuroblastoma cell lines and during neuronal differentiation induced by retinoic acid. Calcyclin gene expression was more frequently detected in epithelial-type or Schwann-like cells rather than in neuroblastic cells. This result indicates an increase of G1 cell fraction, which may explain the limited growth potential usually observed for these cells. LAN-5 cell (neuronal type) differentiation experiments showed that calcyclin gene is detectable after 4 days of retinoic acid treatment, which induces G1 phase accumulation (as detected by cytofluorometric analysis), and cell growth arrest. Otherwise, neither block of cell proliferation by 0.5% fetal calf serum medium nor addition of 15% fresh fetal calf serum after cell arrest induce calcyclin expression. The increase of calcyclin mRNA levels during cell differentiation shows that calcyclin gene expression is associated with neuronal differentiation. This bivalent role of the calcyclin gene, which is normally expressed in the G1 phase of the cell cycle but also expressed during retinoic acid-induced neuroblastoma cell differentiation, suggests that (at least in neuroblastoma cells) the gene is subject to a complex transcriptional regulation.