Diversity, abundance, and sex-specific expression of chemosensory proteins in the reproductive organs of the locust Locusta migratoria manilensis
Diversity, abundance, and sex-specific expression of chemosensory proteins in the reproductive organs of the locust Locusta migratoria manilensis
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东亚飞蝗 (Locusta migratoria manilensis) 生殖器官中化学感应蛋白的多样性、丰度和性别特异性表达
DOI：
10.1515/hsz-2012-0114
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发表时间：
2013
影响因子：
3.7
通讯作者：
Pelosi, Paolo
中科院分区：
文献类型：
--
作者：
Dani, Francesca Romana;Ban, Li-Ping;Iovinella, Immacolata;Zhao, Li-Jing;Gao, Qian;Felicioli, Antonio;Sagona, Simona;Pieraccini, Giuseppe;Pelosi, Paolo
摘要 化学感应蛋白（CSP）是一种小的可溶性蛋白质，通常与昆虫的化学感应器官相关，但也包括参与其他功能的成员，例如信息素传递和发育。尽管感觉器官的 CSP 已被广泛研究，但对其在身体其他部位的功能知之甚少。对可用数据库的首次筛选已确定了东方蝗虫 Locusta migratoria manilensis 中编码 CSP 的 70 个序列。应用蛋白质组学分析，我们鉴定出其中 17 个在女性生殖器官中大量表达，但只有一种 (CSP91) 在男性器官中表达。细菌表达的 CSP91 可特异性结合油酸和亚油酸以及中等长度的醇和酯。在两性生殖器官的二氯甲烷提取物中检测到相同的酸作为主要气相色谱峰。女性生殖器官中 CSP 的丰度和数量表明这些蛋白质的重要作用。我们不能排除 17 个 CSP 中的每一个都可能具有不同的功能，包括化学信息素的传递、激素的溶解、发育的直接控制或其他未知的任务。
Abstract Chemosensory proteins (CSPs) are small soluble proteins often associated with chemosensory organs in insects but include members involved in other functions, such as pheromone delivery and development. Although the CSPs of the sensory organs have been extensively studied, little is known on their functions in other parts of the body. A first screening of the available databases has identified 70 sequences encoding CSPs in the oriental locust Locusta migratoria manilensis. Applying proteomic analysis, we have identified 17 of them abundantly expressed in the female reproductive organs, but only one (CSP91) in male organs. Bacterially expressed CSP91 binds fatty acids with a specificity for oleic and linoleic acid, as well as medium-length alcohols and esters. The same acids have been detected as the main gas chromatographic peaks in the dichloromethane extracts of reproductive organs of both sexes. The abundance and the number of CSPs in female reproductive organs indicates important roles for these proteins. We cannot exclude that different functions can be associated with each of the 17 CSPs, including delivery of semiochemicals, solubilization of hormones, direct control of development, or other unknown tasks.