IRAK-M in macrophages around septically and aseptically loosened hip implants.
IRAK-M in macrophages around septically and aseptically loosened hip implants.
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DOI：
10.1002/jbm.a.33258
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发表时间：
2012
期刊：
影响因子：
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通讯作者：
Yangchun Zhang;C. Hou;Shiming Yu;Jianhong Xiao;Ziji Zhang;Qiyi Zhai;Jianwei Chen;Ziqing Li;Xibao Zhang;M. Lehto;Y. Konttinen;P. Sheng
中科院分区：
文献类型：
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作者：
Yangchun Zhang;C. Hou;Shiming Yu;Jianhong Xiao;Ziji Zhang;Qiyi Zhai;Jianwei Chen;Ziqing Li;Xibao Zhang;M. Lehto;Y. Konttinen;P. Sheng
关节置换术最常见的长期并发症是松动，松动是由植入物产生的碎片刺激巨噬细胞产生的慢性炎症细胞因子介导的，最终细菌成分会附着在这些碎片上。本研究采用免疫组织化学方法和逆转录聚合酶链式反应技术，研究了体内界膜巨噬细胞和钛颗粒刺激的巨噬细胞中抗炎因子-1受体相关激酶-M(IRAK-M)的作用。结果表明，骨性关节炎患者假体界面膜上有较多的IRAK-M蛋白表达，颗粒刺激后IRAK-M基因表达水平升高。这些发现表明，巨噬细胞中IRAK-M的上调参与了种植体周围的局部免疫抑制，并可能导致败血症和无菌性种植体的松动。
The most common long-term complication of joint arthroplasty is loosening, which is mediated by chronic inflammatory cytokines produced by macrophages stimulated by implant-derived debris and eventually bacterial components adherent to such debris. In this study, antiinflammatory interleukin-1 receptor-associated kinase-M (IRAK-M) was studied in macrophages in interface membranes in vivo using immunohistochemical staining and in titanium particle-stimulated macrophages in vitro using reverse transcriptase-polymerase chain reaction. Results show that the interface membranes of septically and aseptically loosened prosthesis express more IRAK-M protein than control membranes from osteoarthritic patient and that IRAK-M mRNA-levels increase upon particle stimulation. These findings suggest that, the upregulation of IRAK-M in macrophages is involved in the local immunosuppression around implants, and may contribute to septic and aseptic implant loosening.