Targeted expression of tetanus neurotoxin interferes with behavioral responses to sensory input in Drosophila
Targeted expression of tetanus neurotoxin interferes with behavioral responses to sensory input in Drosophila
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DOI：
10.1002/neu.10029
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发表时间：
2002-02-15
期刊：
影响因子：
--
通讯作者：
Heisenberg, M
中科院分区：
文献类型：
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作者：
Keller, A;Sweeney, ST;Heisenberg, M
通过表达毒性基因产物靶向灭活神经元是将行为功能分配给特定神经元或脑结构的有用工具。在测试的多种有毒基因产物中，破伤风神经毒素轻链（TNT）的副作用最小，可以完全阻断化学突触。通过使用GAL4系统来驱动TNT在化学和机械感觉神经元的子集中的表达，我们检测到与相关感觉信息的阻断一致的行走和飞行缺陷。我们还发现，第一次，嗅觉行为表型与特定的触角化学感受器的子集的阻断。类似的行为实验与GAL4线在不同的触角化学感受器的子集表达，应有助于了解果蝇的嗅觉编码。为了提高GAL 4系统用于此类目的的实用性，我们设计了一种诱导型系统，可以避免早期发育期间TNT表达引起的致命性。(C)2002年John Wiley Sons，Inc.
Targeted inactivation of neurons by expression of toxic gene products is a useful tool to assign behavioral functions to specific neurons or brain structures. Of a variety of toxic gene products tested, tetanus neurotoxin light chain (TNT) has the least severe side effects and can completely block chemical synapses. By using the GAL4 system to drive TNT expression in a subset of chemo- and mechanosensory neurons, we detected walking and flight defects consistent with blocking of relevant sensory information. We also found, for the first time, an olfactory behavioral phenotype associated with blocking of a specific subset of antennal chemoreceptors. Similar behavioral experiments with GAL4 lines expressing in different subsets of antennal chemoreceptors should contribute to an understanding of olfactory coding in Drosophila. To increase the utility of the GAL4 system for such purposes, we have designed an inducible system that allows as to circumvent lethality caused by TNT expression during early development. (C) 2002 John Wiley Sons, Inc.