Interleukin-32A Cytokine and Inducer of TNFα
Interleukin-32A Cytokine and Inducer of TNFα
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DOI：
10.1016/s1074-7613(04)00380-2
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发表时间：
2005
期刊：
影响因子：
32.4
通讯作者：
Soohyun Kim;Sun-Young Han;T. Azam;D. Yoon;C. Dinarello
中科院分区：
文献类型：
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作者：
Soohyun Kim;Sun-Young Han;T. Azam;D. Yoon;C. Dinarello
我们描述基因结构、调控、信号转导。以及细胞因子白细胞介素 (IL)-32 的功能。通过转染 IL-18 受体 β 链，IL-18 无反应细胞转化为反应细胞，IL-18 诱导的微阵列显示细胞因子样基因的高表达。尽管 IL-32 与已知细胞因子家族不具有序列同源性，但 IL-32 在 THP-1 单核细胞中诱导各种细胞因子、人 TNFα 和 IL-8，以及在 Raw 巨噬细胞中诱导小鼠 TNFα 和 MIP-2。 IL-32 激活核因子 kappa B (NF-κB) 和 p38 丝裂原激活蛋白激酶的典型细胞因子信号通路。 IL-32 mRNA 在免疫组织而非其他组织中高表达。人IL-32以四种剪接变体存在，来自其他物种的IL-32在数据库中被发现为表达序列标签克隆。在人外周淋巴细胞中，在有丝分裂原刺激后，在人上皮细胞中，由 IFNγ 诱导，在 NK 细胞中，在暴露于 IL-12 和 IL-18 组合后，IL-32 可能在炎症/自身免疫性疾病中发挥作用。
We describe the gene structure, regulation, signal transduction. and functions of a cytokine, interleukin (IL)-32. An IL-18 unresponsive cell was converted to a responsive cell by transfection of the IL-18 receptor β chain, and IL-18-induced microarray revealed high expression of a cytokine-like gene. Although IL-32 does not share sequence homology with known cytokine families, IL-32 induces various cytokines, human TNFα, and IL-8 in THP-1 monocytic cells as well as mouse TNFα and MIP-2 in Raw macrophage cells. IL-32 activates typical cytokine signal pathways of nuclear factor-kappa B (NF-κB) and p38 mitogen-activated protein kinase. IL-32 mRNA is highly expressed in immune tissue rather than other tissues. Human IL-32 exists as four splice variants, and IL-32 from other species were found as expressed sequence tag clones in the databank. Induced in human peripheral lymphocyte cells after mitogen stimulation, in human epithelial cells by IFNγ, and in NK cells after exposure to the combination of IL-12 plus IL-18, IL-32 may play a role in inflammatory/autoimmune diseases.