Mapping protective epitopes in the tick and mosquito subolesin ortholog proteins
Mapping protective epitopes in the tick and mosquito subolesin ortholog proteins
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DOI：
10.1016/j.vaccine.2010.06.021
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发表时间：
2010-07-26
期刊：
影响因子：
5.5
通讯作者：
de la Fuente, Jose
中科院分区：
文献类型：
--
作者：
Prudencio, Carlos R.;Perez de la Lastra, Jose Manuel;de la Fuente, Jose
蜱保护性抗原 subolesin 是昆虫警报的结构和功能直系同源物，昆虫警报是一组进化保守的蛋白质，可调节基因表达，从而影响消化、免疫反应、繁殖和发育等多种细胞过程。在本研究中，我们通过计算模型将肽和噬菌体展示文库扫描分析结果与受保护动物的血清相结合，寻找蜱和蚊子 subolesin ortolog 蛋白中的常见保护性表位，三种不同的表位类型（i）线性 B 细胞表位，（ii）构象表位。 (iii) 鉴定了构象不连续表位。 subolesin 直系同源物中保守保护性表位的确定可能会导致多靶点通用疫苗的开发，旨在控制节肢动物感染和降低载体传播病原体的能力 (C) 2010 Elsevier Ltd. 保留所有权利。
The tick protective antigen, subolesin, is a structural and functional ortholog of Insect alarms, an evolutionary conserved group of proteins that regulate gene expression thus affecting multiple cellular processes such as digestion, Immune response, reproduction and development. In this study, we searched for common protective epitopes in tick and mosquito subolesin ortolog proteins By combining the results of peptide and phage-display libraries scan analysis with sera from protected animals with computational modeling, three different epitope types (i) linear B-cell epitopes, (ii) conformational epitopes. and (iii) conformational discontinuous epitopes were identified. The determination of conserved protective epitopes in subolesin ortologs may lead to the development of a multi-target universal vaccine directed at the control of both arthropod infestations and reduction of vector capacity to transmit pathogens (C) 2010 Elsevier Ltd. All rights reserved.