Circulation of Crimean-Congo Hemorrhagic Fever Virus in the Former Yugoslav Republic of Macedonia Revealed by Screening of Cattle Sera Using a Novel Enzyme-linked Immunosorbent Assay
Circulation of Crimean-Congo Hemorrhagic Fever Virus in the Former Yugoslav Republic of Macedonia Revealed by Screening of Cattle Sera Using a Novel Enzyme-linked Immunosorbent Assay
复制标题
DOI：
10.1371/journal.pntd.0003519
复制
发表时间：
2015-03-01
影响因子：
3.8
通讯作者：
Groschup, Martin H.
中科院分区：
文献类型：
--
作者：
Mertens, Marc;Vatansever, Zati;Groschup, Martin H.
背景：在动物中检测克里米亚-刚果出血热病毒（CCHFV）特异性抗体的检测方法很少，而且大多数检测方法的诊断敏感性和特异性数据记录不完整。这是不幸的，因为动物体内的CCHFV抗体可作为一个地理区域病毒传播的指标，从而具有人类接触的潜在风险。因此，本文报道了一种检测牛体内cchfv特异性抗体的新型酶联免疫吸附试验及其在检测前南斯拉夫马其顿共和国反刍动物血清中的应用。主要发现建立了一种灵敏、特异的ELISA检测牛体内cchfv特异性IgG抗体。通过使用来自一个迄今从未检测到CCHFV的国家的503份阴性血清样本和54份阳性血清样本，验证了该检测方法。使用两种市售的测定法（用于检测人血清）对阳性血清进行了验证，我们已将这两种测定法适用于动物。该方法灵敏度为98%，特异度为99%。在前南斯拉夫的马其顿共和国证实了蜱虫的存在，根据区域不同，在牛群中检测到高达80%的抗体流行率。结论建立了一种检测牛体内cchfv特异性IgG抗体的酶联免疫吸附试验。利用这一检测方法，首次在前南斯拉夫的马其顿共和国的牛中检测到cchfv特异性抗体，为该病毒在该国的活跃传播提供了证据。支持这一结论的是，本工作首次在前南斯拉夫的马其顿共和国证实了CCHFV主要病媒的存在。
BackgroundThere are only few assays available for the detection of Crimean-Congo Hemorrhagic Fever Virus (CCHFV)-specific antibodies in animals, and data about diagnostic sensitivity and specificity are incompletely documented for most of these tests. This is unfortunate since CCHFV antibodies in animals can be used as indicator for virus circulation in a geographic area and therewith potential risk of human exposure. This paper therefore reports on a novel ELISA for the detection of CCHFV-specific antibodies in cattle and on its application for testing ruminant sera from the Former Yugoslav Republic of Macedonia.Principal FindingsA highly sensitive and specific ELISA was developed to detect CCHFV-specific IgG antibodies in cattle. The assay was validated by using 503 negative serum samples from a country where CCHFV has never been detected until now, and by using 54 positive serum samples. The positive sera were verified by using two commercially available assays (for testing human serum) which we have adapted for use in animals. The sensitivity of the novel ELISA was 98% and its specificity 99%. The presence of Hyalomma ticks was demonstrated in the Former Yugoslav Republic of Macedonia and depending on the region antibody prevalence rates up to 80% were detected in the cattle population.ConclusionThis article describes a fully validated, highly sensitive and specific ELISA for the detection of CCHFV-specific IgG antibodies in cattle. Using this assay, CCHFV-specific antibodies were detected for the first time in cattle in the Former Yugoslav Republic of Macedonia, giving evidence for an active circulation of this virus in the country. Supporting this conclusion, the occurrence of the main vector of CCHFV was demonstrated in the present work for the first time in Former Yugoslav Republic of Macedonia.