Small-angle neutron scattering and contrast variation: a powerful combination for studying biological structures
Small-angle neutron scattering and contrast variation: a powerful combination for studying biological structures
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DOI：
10.1107/s090744491001
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发表时间：
2010-11-01
期刊：
影响因子：
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通讯作者：
Heller, William T.
中科院分区：
文献类型：
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作者：
Heller, William T.
小角散射（SAS）在生物科学中的使用继续增加，这是由于需要研究越来越复杂的系统，这些系统通常抗结晶或对于NMR来说太大，以及用户设施的可用性和SAS数据生物结构建模的进步。SAS，无论是中子（SANS）还是X射线（SAXS），都是长度范围从10到10 000 A的结构探针。当应用于稀释溶液中的生物复合物时，它提供了大小和形状信息，可用于产生结构模型，从而可以提供对功能的洞察。SANS通过中子与氢及其同位素氘的独特相互作用实现对比度变化方法的使用。具有对比度变化的SANS使多亚基复合物内的组分可视化，使其成为探测蛋白质-蛋白质和蛋白质-核酸复合物以及蛋白质与脂质和去污剂相互作用的有力工具。
The use of small-angle scattering (SAS) in the biological sciences continues to increase, driven as much by the need to study increasingly complex systems that are often resistant to crystallization or are too large for NMR as by the availability of user facilities and advancements in the modelling of biological structures from SAS data. SAS, whether with neutrons (SANS) or X-rays (SAXS), is a structural probe of length scales ranging from 10 to 10 000 A. When applied to biological complexes in dilute solution, it provides size and shape information that can be used to produce structural models that can provide insight into function. SANS enables the use of contrast-variation methods through the unique interaction of neutrons with hydrogen and its isotope deuterium. SANS with contrast variation enables the visualization of components within multisubunit complexes, making it a powerful tool for probing protein-protein and protein-nucleic acid complexes, as well as the interaction of proteins with lipids and detergents.