Wireless Optofluidic Systems for Programmable In Vivo Pharmacology and Optogenetics.
Wireless Optofluidic Systems for Programmable In Vivo Pharmacology and Optogenetics.
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DOI：
10.1016/j.cell.2015.06.058
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发表时间：
2015-07-30
期刊：
影响因子：
64.5
通讯作者：
Rogers JA
中科院分区：
文献类型：
--
作者：
Jeong JW;McCall JG;Shin G;Zhang Y;Al-Hasani R;Kim M;Li S;Sim JY;Jang KI;Shi Y;Hong DY;Liu Y;Schmitz GP;Xia L;He Z;Gamble P;Ray WZ;Huang Y;Bruchas MR;Rogers JA
体内药理学和光遗传学在剖析清醒、行为动物的神经回路、细胞信号和操纵神经生理系统方面有着巨大的希望。现有的神经接口技术，如用于药物输注的连接到外部药物供应的金属套管和用于光遗传学的系留光纤，对于对自由行为的动物进行微创、自由行为的研究并不理想。在这里，我们介绍了无线光流控神经探针，它将超薄、柔软的微流控药物输送与细胞规模的无机发光二极管(μ)阵列相结合。这些探头比套管小几个数量级，允许对液体输送和光刺激进行无线、程序化的时空控制。我们在自由活动的动物中展示了这些装置来改变基因表达，递送多肽配体，并提供与拮抗剂药物递送同步的光刺激，以操纵Mesovavbens奖赏相关行为。这些探针的微创操作预示着在其他器官系统和物种中的应用，在生物医学科学、工程和医学中具有广泛的应用潜力。
In vivo pharmacology and optogenetics hold tremendous promise for dissection of neural circuits, cellular signaling and manipulating neurophysiological systems in awake, behaving animals. Existing neural interface technologies, such as metal cannulas connected to external drug supplies for pharmacological infusions and tethered fiber optics for optogenetics, are not ideal for minimally-invasive, untethered studies on freely behaving animals. Here we introduce wireless optofluidic neural probes that combine ultrathin, soft microfluidic drug delivery with cellular-scale inorganic light-emitting diode (μ-ILED) arrays. These probes are orders of magnitude smaller than cannulas and allow wireless, programmed spatiotemporal control of fluid delivery and photostimulation. We demonstrate these devices in freely moving animals to modify gene expression, deliver peptide ligands, and provide concurrent photostimulation with antagonist drug delivery to manipulate mesoaccumbens reward-related behavior. The minimally-invasive operation of these probes forecasts utility in other organ systems and species, with potential for broad application in biomedical science, engineering, and medicine.