Caspase-1-Dependent Pyroptosis of Peripheral Blood Mononuclear Cells Is Associated with the Severity and Mortality of Septic Patients
Caspase-1-Dependent Pyroptosis of Peripheral Blood Mononuclear Cells Is Associated with the Severity and Mortality of Septic Patients
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Caspase-1 依赖性外周血单核细胞焦亡与脓毒症患者的严重程度和死亡率相关
DOI：
10.1155/2020/9152140
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发表时间：
2020-02-29
影响因子：
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通讯作者：
Li, Zhanfei
中科院分区：
文献类型：
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作者：
Wang, Yuchang;Liu, Yukun;Li, Zhanfei
目的：研究发现，在感染、损伤或炎症性疾病引起的炎症过程中，焦亡起着至关重要的作用。本研究旨在评估脓毒症患者外周血单个核细胞（PBMC）焦亡的百分比，并评估PBMC焦亡与脓毒症患者严重程度和死亡率的相关性。方法对128例创伤后脓毒症患者进行前瞻性队列研究。在诊断脓毒症后24小时内收集血液样品，并通过流式细胞术测量PBMC焦亡。结果PBMC焦亡率与急性生理学和慢性健康状况评分（APACHE）II评分及序贯器官衰竭评分（SOFA）呈正相关（均P < 0.01）。受试者操作特征曲线上PBMC焦亡百分比的曲线下面积（AUC）为0.79（95%置信区间（CI），0.68-0.90）。考克斯比例风险模型确定了PBMC焦亡百分比增加（>14.17%）与28天死亡率风险增加之间的相关性（风险比= 1.234，95% CI，1.014-1.502）。结论脓毒症患者外周血单个核细胞（PBMC）凋亡率增高，PBMC凋亡率增高与脓毒症的严重程度及脓毒症患者28 d病死率有关。
Purpose Pyroptosis has been known to play a vital role in the inflammation process which was induced by infection, injury, or inflammatory disease. The present study was aimed at evaluating the percentage of peripheral blood mononuclear cell (PBMC) pyroptosis in septic patients and assessing the correlation of PBMC pyroptosis with the severity and the mortality of septic patients. Methods 128 trauma-induced patients with sepsis were enrolled in this prospective cohort study. Blood samples were collected, and PBMC pyroptosis was measured by flow cytometry within 24 hours after sepsis was diagnosed. Results Percentage of PBMC pyroptosis was positively correlated with the acute physiology and chronic health evaluation (APACHE) II score and sequential organ failure assessment (SOFA) score (all P < 0.01). The area under the curve (AUC) for the percentage of PBMC pyroptosis on a receiver operating characteristic curve was 0.79 (95% confidence interval (CI), 0.68–0.90). A Cox proportional hazard model identified an association between an increased percentage of PBMC pyroptosis (>14.17%) and increased risk of the 28-day mortality (hazard ratio = 1.234, 95% CI, 1.014–1.502). Conclusion The percentage of PBMC pyroptosis increases in septic patients, and the increased percentage of PBMC pyroptosis is associated with the severity of sepsis and the 28-day mortality of patients with sepsis.