Concurrent upregulation of postsynaptic L-type Ca2+ channel function and protein kinase A signaling is required for the periadolescent facilitation of Ca2+ plateau potentials and dopamine D1 receptor modulation in the prefrontal cortex
Concurrent upregulation of postsynaptic L-type Ca2+ channel function and protein kinase A signaling is required for the periadolescent facilitation of Ca2+ plateau potentials and dopamine D1 receptor modulation in the prefrontal cortex
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DOI：
10.1016/j.neuropharm.2011.01.041
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发表时间：
2011-05-01
影响因子：
4.7
通讯作者：
Tseng, Kuei Y.
中科院分区：
文献类型：
--
作者：
Heng, Li-Jun;Markham, Julie A.;Tseng, Kuei Y.
进一步了解前额皮质（PFC）回路在出生后发育过程中如何变化引起了人们的极大兴趣，因为它在工作记忆和决策中发挥着重要作用，这两种认知能力在青春期后期得到完善，并在精神分裂症中发生改变。虽然很明显，多巴胺促进谷氨酸反应发生在青春期的前额皮质中，但我们对支持这些变化的细胞机制知之甚少。其中，突触后 Ca2+ 功能的发育促进特别令人感兴趣，因为它在协调神经元整体中发挥着作用，这一过程被认为有助于 PFC 功能的成熟。在这里，我们对 PFC 脑切片中的深层锥体神经元进行全细胞膜片钳记录，并确定体细胞诱发的 Ca2+ 介导的平台去极化在出生后第 25 天（青少年）到成年（PD 80）期间如何变化。 PFC 中的突触后 Ca2+ 电位在整个出生后发育过程中持续时间增加。 PD 40 后观察到从短去极化到长时间去极化的显着转变。这种变化反映在 L 型 Ca2+ 通道功能和突触后 PKA 信号传导的增强。我们推测，PFC 中 Ca2+ 反应的长期发育促进可能有助于改善青春期的工作记忆性能。 (C) 2011 Elsevier Ltd. 保留所有权利。
Further understanding of how prefrontal cortex (PFC) circuit change during postnatal development is of great interest due to its role in working memory and decision-making, two cognitive abilities that are refined late in adolescence and become altered in schizophrenia. While it is evident that dopamine facilitation of glutamate responses occurs during adolescence in the PFC, little is known about the cellular mechanisms that support these changes. Among them, a developmental facilitation of postsynaptic Ca2+ function is of particular interest given its role in coordinating neuronal ensembles, a process thought to contribute to maturation of PFC function. Here we conducted whole-cell patch clamp recordings of deep-layer pyramidal neurons in PFC brain slices and determined how somatic-evoked Ca2+-mediated plateau depolarizations change throughout postnatal day (PD) 25 (juvenile) to adulthood (PD 80). Postsynaptic Ca2+ potentials in the PFC increase in duration throughout postnatal development. A remarkable shift from short to prolonged depolarizations was observed after PD 40. This change is reflected by an enhancement of L-type Ca2+ channel function and postsynaptic PKA signaling. We speculate that such a protracted developmental facilitation of Ca2+ response in the PFC may contribute to improvement of working memory performance through adolescence. (C) 2011 Elsevier Ltd. All rights reserved.