Comparison of genomic and predicted amino acid sequences of respiratory and enteric bovine coronaviruses isolated from the same animal with fatal shipping pneumonia
Comparison of genomic and predicted amino acid sequences of respiratory and enteric bovine coronaviruses isolated from the same animal with fatal shipping pneumonia
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DOI：
10.1099/0022-1317-82-12-2927
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发表时间：
2001-12-01
影响因子：
3.8
通讯作者：
Gorbalenya, AE
中科院分区：
文献类型：
--
作者：
Chouljenko, VN;Lin, XQ;Gorbalenya, AE
这里报告了两种牛冠状病毒（BCoV）现场分离株的完整基因组序列，这些序列是从在牛运输热流行期间经历致命性肺炎的同一动物的呼吸道和肠道样本中分离出来的。两个基因组均含有 31028 个核苷酸，并包含 13 个开放阅读框 (ORF)，两侧为 5'- 和 3'- 非翻译区 (UTR)。 ORF1a 和 ORF1b 分别编码复制多蛋白 pp1a 和 pp1ab，它们包含先前记录的最近亲小鼠肝炎病毒的所有推定功能域。 BCoV 分离株的基因组有 107 个位置不同，分布在除 5'-UTR 之外的整个基因组中。 25 个位置的差异是非同义的，并且存在于除 pp 1 b 之外的所有蛋白质中。在预测的最大 pp1a 衍生蛋白 p195/p210 内或紧邻其下游发现了 6 个复制酶突变。 p195/p210 以及 S 糖蛋白内的单个氨基酸变化可能导致 BCoV 分离株的不同表型。
The complete genome sequences are reported here of two field isolates of bovine coronavirus (BCoV), which were isolated from respiratory and intestinal samples of the same animal experiencing fatal pneumonia during a bovine shipping fever epizootic. Both genomes contained 31028 nucleotides and included 13 open reading frames (ORFs) flanked by 5'- and 3'-untranslated regions (UTRs). ORF1a and ORF1 b encode replicative polyproteins pp1a and pp1ab, respectively, that contain all of the putative functional domains documented previously for the closest relative, mouse hepatitis virus. The genomes of the BCoV isolates differed in 107 positions, scattered throughout the genome except the 5'-UTR. Differences in 25 positions were non-synonymous and were located in all proteins except pp 1 b. Six replicase mutations were identified within or immediately downstream of the predicted largest pp1a-derived protein, p195/p210. Single amino acid changes within p195/p210 as well as within the S glycoprotein might contribute to the different phenotypes of the BCoV isolates.