Characterization of high molecular weight impurities in synthetic phosphorothioate oligonucleotides
Characterization of high molecular weight impurities in synthetic phosphorothioate oligonucleotides
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DOI：
10.1016/j.bmcl.2005.10.051
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发表时间：
2006-02-01
影响因子：
2.7
通讯作者：
Capaldi, DC
中科院分区：
文献类型：
--
作者：
Kurata, C;Bradley, K;Capaldi, DC
通过标准亚磷酰胺技术使用2 '-脱氧腺苷-或2'-O-（2-甲氧基乙基）-5-甲基胞嘧啶-负载的固体支持物生产的硫代磷酸酯寡核苷酸含有支链杂质，由两条链组成，其中一条链通过3 '-末端核苷的环外氨基连接，另一条链通过P（O）SH基团连接3'-末端羟基。当使用通用的非核苷衍生支持物时，不会产生这些杂质。(c)2005爱思唯尔有限公司保留所有权利。
Phosphorothioate oligonucleotides manufactured by standard phosphoramidite techniques using 2'-deoxyadenosine- or 2'-O-(2-methoxyethyl)-5-methylcytosine-loaded solid supports contain branched impurities consisting of two chains linked through the exocyclic amino group of the 3'-terminal nucleoside of one chain and the 3'-terminal hydroxyl group of another via a P(O)SH group. These impurities are not produced when a universal, non-nucleoside derivatized support is used. (c) 2005 Elsevier Ltd. All rights reserved.