Regulation of flowering time by the protein arginine methyltransferase AtPRMT10
Regulation of flowering time by the protein arginine methyltransferase AtPRMT10
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DOI：
10.1038/sj.embor.7401111
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发表时间：
2007-12-01
期刊：
影响因子：
7.7
通讯作者：
Cao, Xiaofeng
中科院分区：
文献类型：
--
作者：
Niu, Lifang;Lu, Falong;Cao, Xiaofeng
在植物中，组蛋白H3赖氨酸甲基转移酶在基因沉默和发育调控中具有重要作用，然而，组蛋白H4甲基化在植物发育中的作用仍不清楚。最近的研究发现了一种II型组蛋白精氨酸甲基转移酶AtPRTM 5，它参与促进生长和开花。在这里，我们纯化了二聚化的植物特异性组蛋白H4甲基转移酶，植物组蛋白精氨酸甲基转移酶10（PHRMT 10），从花椰菜。拟南芥蛋白质精氨酸甲基转移酶10（AtPRMT 10）-PHRMT 10的拟南芥同源物-被证明是1型PRMT，其在体外优先不对称甲基化组蛋白H4 R3。AtPRMT 10的遗传破坏通过上调FLC转录水平导致开花延迟。此外，我们发现AtPRMT 10的功能与AtPRMT 5在遗传上是分开的，但每个功能都可以微调FLC的表达。这项工作为开花时间调控增加了额外的复杂性，也揭示了不对称精氨酸甲基化在植物发育中的重要性。
In plants, histone H3 lysine methyltransferases are important in gene silencing and developmental regulation; however, the roles of histone H4 methylation in plant development remain unclear. Recent studies found a type II histone arginine methyltransferase, AtPRTM5, which is involved in promoting growth and flowering. Here, we purified a dimerized plant-specific histone H4 methyltransferase, plant histone arginine methyltransferase 10 (PHRMT10), from cauliflower. Arabidopsis thaliana protein arginine methyltransferase 10 (AtPRMT10)-the Arabidopsis homologue of PHRMT10-was shown to be a type 1 PRMT, which preferentially asymmetrically methylated histone H4R3 in vitro. Genetic disruption of AtPRMT10 resulted in late flowering by upregulating FLOWERING LOCUS C (FLC) transcript levels. In addition, we show that AtPRMT10 functions genetically separate from AtPRMT5, but that each acts to fine-tune expression of FLC. This work adds an extra layer of complexity to flowering-time regulation and also sheds light on the importance of asymmetric arginine methylation in plant development.