Phylogenetic distribution and predominant genotype of the avian infectious bronchitis virus in China during 2008-2009.
Phylogenetic distribution and predominant genotype of the avian infectious bronchitis virus in China during 2008-2009.
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DOI：
10.1186/1743-422x-8-184
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发表时间：
2011-04-22
期刊：
影响因子：
4.8
通讯作者：
Xie Q
中科院分区：
文献类型：
--
作者：
Ji J;Xie J;Chen F;Shu D;Zuo K;Xue C;Qin J;Li H;Bi Y;Ma J;Xie Q
2008-2009年肾型鸡传染性支气管炎给我国商品养鸡业造成了前所未有的经济损失。为了解肾型传染性支气管炎（IBV）在我国的流行情况，对2008-2009年从我国不同省份分离的80株IBV分离株进行矮胚试验和RT-PCR鉴定。菌株主要在冬春季分离，发病年龄范围广，从4天到69天。经病毒恢复试验，70/80的病毒株可导致鸟类因肾炎而死亡或痛苦。为了解流行毒株的分子进化特征，采用RT-PCR方法对流行毒株的主要刺突蛋白1（major spike 1，S1）基因编码区进行了扩增和序列测定。与已发表的代表菌株进行核苷酸和氨基酸序列分析，结果表明，这些菌株与参考菌株的S1基因同源性分别为75.1%~ 99.8%和73.1%~ 99.8%。主要流行株S1蛋白含有540或542个氨基酸，具有HRRRR或RRFRR切割位点。系统进化分析表明，我国近年来分离的IBV主要属于A2-分支（QXIBV-分支）和HN 08-分支，仅有1株分别属于Gray-分支和M41-分支。80株中的大多数与疫苗株在进化上有一定的距离。本研究结果提示，2008-2009年我国肾致病性IBV主要为A2类毒株。
The nephropathogenic avian infectious bronchitis (IB) caused unprecedented economic losses to the commercial chicken industry of China in 2008-2009. To investigate the prevalence of nephropathogenic IB in China, eighty IBV isolates from different provinces during 2008-2009 were identified by dwarf embryo test and RT-PCR. The strains were mostly isolated in winter and spring with a wide age range of IB outbreaks, from 4 to 69 days. By the virus recovery trials, 70/80 of the strains resulted in the deaths or distresses of birds from nephritis. To learn more about the molecular evolutionary characteristics of the circulating field strains, the coding region of major spike 1 (S1) protein gene of these strains was RT-PCR amplified and sequenced. Compared to the published representative strains, nucleotides and amino acids sequence analysis indicated that the S1 genes of these strains and the reference strains displayed homologies ranging from 75.1% to 99.8% and from 73.1% to 99.8% respectively. S1 protein of the major pandemic strains contained 540 or 542 amino acids with the cleavage site of HRRRR or RRFRR. Phylogenetic analysis revealed that recent field isolates of IBV in China were mostly belonged to A2-branch (QXIBV-branch) and HN08-branch, only one isolate was belonged to Gray-branch and M41-branch respectively. Most of the 80 strains showed evolutionarily distant from vaccine strains. The results of this study suggested that nephropathogenic IBVs were mainly A2-like strains in China during 2008-2009.