Erythropoietin and erythropoietin receptor expression in the guinea pig inner ear
Erythropoietin and erythropoietin receptor expression in the guinea pig inner ear
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DOI：
10.1016/j.heares.2004.11.017
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发表时间：
2005-05-01
期刊：
影响因子：
2.8
通讯作者：
Thomsen, J
中科院分区：
文献类型：
--
作者：
Cayé-Thomasen, P;Wagner, N;Thomsen, J
促红细胞生成素受体（EPOR）在脑中表达，并且促红细胞生成素（EPO）已显示在中枢神经系统和视网膜中具有神经营养和神经保护功能。这些发现可能适用于内耳，等待EPO受体的存在。因此，本研究确定了豚鼠内耳中EPO和EPOR的表达。正常豚鼠内耳进行免疫组织化学处理，使用多克隆抗体对EPO和EPO受体。EPO表达仅见于大多数螺旋神经节神经元，但不是所有螺旋神经节神经元。EPO受体的表达见于内和外指骨细胞（Deiters细胞）以及内沟细胞和Corti器官的支持细胞（Hensen、Claudius和一些Boettcher细胞）的细胞质中。部分螺旋神经节神经元或神经胶质细胞表达该受体，螺旋韧带纤维细胞、血管纹中间细胞和蜗轴血管内皮细胞也表达该受体。前庭系统的任何部分均未对任一抗体染色呈阳性。我们的结论是，EPO是由螺旋神经节神经元和EPO受体广泛表达的几种细胞类型内的豚鼠耳蜗。我们假设存在局部旁分泌系统，并且在内耳损伤后EPO治疗可能是可行的。(c)2004年由Elsevier B.V.出版
The erythropoietin receptor (EPOR) is expressed in the brain and erythropoietin (EPO) has been shown to have neurotrophic and neuroprotective functions in the central nervous system and in the retina. These findings may be applied to the inner ear, pending EPO receptor presence. Accordingly, this study determines expression of EPO and EPOR in the inner ear of the guinea pig. Normal guinea pig inner ears were processed for immunohistochemistry, using poly-clonal antibodies against EPO and the EPO receptor. EPO expression was exclusively found in most, but not all spiral ganglion neurons. Expression of the EPO receptor was found in the cytoplasm of the inner and outer phalangeal cells (Deiters' cells), as well as the inner sulcus cells and the supporting cells of the organ of Corti (Hensen, Claudius and some Boettcher cells). Some spiral ganglion neurons or glial cells expressed the receptor, as did spiral ligament fibrocytes, some intermediate cells of stria vascularis and the endothelial cells of some modiolar vessels. No parts of the vestibular system stained positive for either antibody. We conclude, that EPO is expressed by spiral ganglion neurons and that the EPO receptor is widely expressed by several cell types within the guinea pig cochlea. We hypothesize on the existence of a local paracrine system and that EPO treatment may be feasible following inner ear damage. (c) 2004 Published by Elsevier B.V.