High virus titer in feather pulp of chickens infected with subgroup J avian leukosis virus
High virus titer in feather pulp of chickens infected with subgroup J avian leukosis virus
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DOI：
10.1637/0005-2086(2002)046
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发表时间：
2002-04-01
期刊：
影响因子：
1.4
通讯作者：
Fadly, AM
中科院分区：
文献类型：
--
作者：
Sung, HW;Reddy, SM;Fadly, AM
J亚群禽白血病病毒（ALV）是介于外源性和内源性ALV之间的重组病毒，可通过垂直或水平传播。在羽髓中可以检测到外源和内源ALV。本研究对感染J亚群禽白血病病毒的鸡羽髓、血浆和泄殖腔拭子中病毒滴度进行了比较。1日龄接种J亚群禽白血病病毒的肉鸡，在2 ~ 8周龄的试验期间，羽髓病毒均呈阳性。部分感染J亚群ALV的肉鸡羽髓中病毒滴度很高，为107 ~ 101感染单位/0.2ml。在血浆、泄殖腔拭子和羽浆样品中，羽浆中的病毒滴度通常最高。在另一个试验中，用J亚群ALV在1日龄接种蛋鸡，从2周龄到18周龄，在羽髓中检测到病毒，并且病毒在羽髓中的持续时间比在血浆中的持续时间长。从2、4、10周龄羽髓中提取的DNA进行聚合酶链反应（PCR）检测，几乎所有的蛋鸡均呈阳性，且羽髓PCR的敏感性高于血浆、泄殖腔拭子和羽髓分离的病毒。上述结果表明，羽毛髓标本可用于病毒分离和PCR检测，以确认J亚群ALV感染。
Subgroup J avian leukosis viruses (ALVs), which are a recombinant virus between exogenous and endogenous ALVs, can spread by either vertical or horizontal transmission. Exogenous and endogenous ALVs can be detected in feather pulp. In this study, virus titers in feather pulp of chickens infected with subgroup J ALV were compared with those of plasma and cloacal swab. All of the broiler chickens inoculated with subgroup J ALV at I day old were positive for virus from feather pulp during the experimental period of between 2 wk and 8 wk of age. Virus titers in feather pulp of some broiler chickens infected with subgroup J ALV were very high, ranging from 107 to 101 infective units per 0.2 ml. Virus titers in feather pulp were usually the highest among the samples of plasma, cloacal swab, and feather pulp tested. In another experiment in which layer chickens were inoculated with subgroup J ALV at I day old, virus was detected in feather pulp from 2 wk until 18 wk of age, and Virus persisted longer in feather pulp than in plasma. Almost all of the layer chickens tested were positive for virus by polymerase chain reaction (PCR) with DNA extracted from feather Pulp samples at 2, 4, and 10 wk of age, and the PCR from feather pulp was more sensitive than virus isolation from Plasma, cloacal swab, and feather pulp. All above results indicate that samples of feather pulp can be useful for virus isolation and PCR to confirm subgroup J ALV infection.