Online nanoelectrospray/high-field asymmetric waveform ion mobility spectrometry as a potential tool for discovery pharmaceutical bioanalysis
Online nanoelectrospray/high-field asymmetric waveform ion mobility spectrometry as a potential tool for discovery pharmaceutical bioanalysis
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DOI：
10.1002/rcm.4311
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发表时间：
2009-12-01
影响因子：
2
通讯作者：
Wu, Jing-Tao
中科院分区：
文献类型：
--
作者：
Hatsis, Panos;Valaskovic, Gary;Wu, Jing-Tao
研究了纳米电喷雾电离（nESI）与高场非对称波形离子迁移谱（FAIMS）在线耦合用于发现药物环境中的小分子分析。使用常规毛细管泵、自动进样器和nESI源将样品直接引入FAIMS装置中。FAIMS装置用于在与质谱仪兼容的时间尺度上分离气相离子。证明了nESI-FAIMS组合有效去除母体药物代谢物干扰并降低离子抑制效应的能力。平均而言，在提取的血浆样品中保留了从纯样品获得的85%的信号强度。制备了几种化合物的标准曲线。在约3至4个数量级内获得线性。比较nESI-FAIMS与常规LC/MS在小鼠药代动力学研究中的结果，得到的浓度值差异不超过30%。版权所有（c）2009约翰威利父子有限公司。
Nanoelectrospray ionization (nESI) coupled online with high-field asymmetric waveform ion mobility spectrometry (FAIMS) for small molecule analysis in a discovery pharmaceutical setting was examined. A conventional capillary pump, autosampler and nESI source were used to introduce samples directly into the FAIMS device, The FAIMS device was used to separate gas-phase ions on a timescale that was compatible with the mass spectrometer. The capability of the nESI-FAIMS combination to efficiently remove metabolite interferences from the parent drug, and reduce ion suppression effects, was demonstrated. On average, 85% of the signal intensity obtained from a neat sample was preserved in the extracted plasma samples. Standard curves were prepared for several compounds. Linearity was obtained over approximately 3 to 4 orders of magnitude. Comparison of results from nESI-FAIMS with those from conventional LC/MS for a mouse pharmacokinetic study yielded concentration values differing by no more than 30%. Copyright (c) 2009 John Wiley & Sons, Ltd.