Calcium signalling mediates self-incompatibility response in the Brassicaceae
Calcium signalling mediates self-incompatibility response in the Brassicaceae
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钙信号介导十字花科植物的自交不亲和反应
DOI：
10.1038/nplants.2015.143
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发表时间：
2015
期刊：
影响因子：
18
通讯作者：
Takayama Seiji
中科院分区：
文献类型：
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作者：
Iwano M;Ito K;Fujii S;Kakita M;Asano-Shimosato H;Igarashi M;Kaothien-Nakayama P;Entani T;Kanatani A;Takehisa M;Tanaka M;Komatsu K;Shiba H;Nagai T;Miyawaki A;Isogai A;Takayama Seiji
十字花科的自交不亲和性是由编码花粉配体（S-位点蛋白11，SP11，也称为S-位点富含半胱氨酸蛋白，SCR）及其柱头受体（S-受体激酶，SRK）的多个单倍型控制的。 SP11/SCR 和 SRK 之间的单倍型特异性相互作用会触发自体不亲和反应，从而导致自体花粉排斥，但信号通路仍然很大程度上未知。在这里，我们表明 Ca 2+ 流入柱头乳头细胞介导自交不亲和信号传导。使用表达SP11/SCR和SRK的自交不亲和拟南芥，我们发现自花授粉特异性诱导乳头细胞中细胞质Ca 2+ ([Ca 2+] cyt)的增加。直接将 SP11/SCR 应用于乳头细胞原生质体可诱导 Ca 2+ 增加，该增加可被谷氨酸受体通道阻滞剂 D-(−)-2-amino-5-Phosphonopentanoic Acid (AP-5) 抑制。人为增加乳头细胞中的 [Ca 2+] 细胞色素可阻止野生型 (WT) 花粉水合作用。用AP-5处理乳头细胞会干扰自交不亲和性，并且在谷氨酸受体样通道(GLR)基因突变体中，自交不亲和反应中Ca 2+ 的增加减少。这些结果表明，GLR介导的Ca 2+ 流入是导致自体花粉排斥的重要自体不亲和性反应。
Self-incompatibility in the Brassicaceae is controlled by multiple haplotypes encoding the pollen ligand (S-locus protein 11, SP11, also known as S-locus cysteine-rich protein, SCR) and its stigmatic receptor (S-receptor kinase, SRK). A haplotype-specific interaction between SP11/SCR and SRK triggers the self-incompatibility response that leads to self-pollen rejection, but the signalling pathway remains largely unknown. Here we show that Ca 2+ influx into stigma papilla cells mediates self-incompatibility signalling. Using self-incompatible Arabidopsis thaliana expressing SP11/SCR and SRK, we found that self-pollination specifically induced an increase in cytoplasmic Ca 2+([Ca 2+] cyt) in papilla cells. Direct application of SP11/SCR to the papilla cell protoplasts induced Ca 2+ increase, which was inhibited by D-(−)-2-amino-5-phosphonopentanoic acid (AP-5), a glutamate receptor channel blocker. An artificial increase in [Ca 2+] cyt in papilla cells arrested wild-type (WT) pollen hydration. Treatment of papilla cells with AP-5 interfered with self-incompatibility, and Ca 2+ increase on the self-incompatibility response was reduced in the glutamate receptor-like channel (GLR) gene mutants. These results suggest that Ca 2+ influx mediated by GLR is the essential self-incompatibility response leading to self-pollen rejection.