Transcriptionally active c-myc oncogene is contained within NIARD, a DNA sequence associated with chromosome translocations in B-cell neoplasia.
Transcriptionally active c-myc oncogene is contained within NIARD, a DNA sequence associated with chromosome translocations in B-cell neoplasia.
复制标题
NIARD 中包含具有转录活性的 c-myc 癌基因，NIARD 是与 B 细胞肿瘤中染色体易位相关的 DNA 序列。
DOI：
10.1073/pnas.80.2.519
复制
发表时间：
1983
影响因子：
11.1
通讯作者：
Croce,CM
中科院分区：
文献类型：
--
作者：
Marcu,KB;Harris,LJ;Stanton,LW;Erikson,J;Watt,R;Croce,CM
NIARD（非免疫球蛋白相关重排DNA）位于小鼠15号染色体上，在小鼠浆细胞瘤中通常观察到的涉及15号和12号染色体的易位事件的断点处。已知禽骨髓细胞瘤病毒株MC-29的v-肌原基因的人细胞类似物位于人8号染色体的远端，并已观察到在伯基特淋巴瘤中易位至14号染色体。使用的cDNA克隆特异性的转录本的人c-myc基因（H c-myc），我们表明，小鼠c-myc（M c-myc）基因是包含在NIARD。NIARD相关的染色体易位发生在小鼠c-mycgene的1.3 - 2kb（kb）5 ′端，在此NIARD与各种小鼠浆细胞瘤中的C α免疫球蛋白基因的开关区重组。小鼠NIARD中的c-mycen编码区跨越<2.4kb的DNA，并在BALB/c脾中表达低水平的2.3kb多聚腺苷酰化RNA。重排的小鼠c-myctranscripts水平增加（10 - 20倍）（即，在具有NIARD相关染色体易位的浆细胞瘤中观察到约1.8 - 2.1 kb）。人c-mycand NIARD探针检测到7例Burkitt淋巴瘤中4例的人c-mycin DNA重排。在几例Burkitt淋巴瘤中，与人c-mycgene相邻的DNA序列与14号染色体上的C μ免疫球蛋白基因位点重组。染色体易位激活c-myconcogene暗示其参与B细胞肿瘤发生。
NIARD (non-immunoglobulin-associated rearranging DNA) is located on mouse chromosome 15 at the break point of a commonly observed translocation event involving chromosomes 15 and 12 in murine plasmacytomas. The human cellular analogue of the v-myconcogene of avian myelocytomatosis virus, strain MC-29, is known to reside on the distal end of human chromosome 8 and has been observed to translocate to chromosome 14 in Burkitt lymphomas. Using a cDNA clone specific for the transcript of the human c-mycgene (H c-myc), we show that the mouse c-myc(M c-myc) gene is contained within NIARD. NIARD-associated chromosome translocations occurred 1.3-2 kilobases (kb) 5′ of the mouse c-mycgene where NIARD recombines with the switch region of the Cαimmunoglobulin gene in various murine plasmacytomas. The mouse c-mycencoding region within NIARD spanned <2.4 kb of DNA and expressed a low level of a 2.3-kb polyadenylylated RNA in BALB/c spleen. Increased (10- to 20-fold) levels of rearranged mouse c-myctranscripts (i.e., ≈1.8-2.1 kb) were observed in plasmacytomas that have NIARD-associated chromosome translocations. Human c-mycand NIARD probes detected DNA rearrangements of human c-mycin four of seven Burkitt lymphomas. DNA sequences adjacent to the human c-mycgene recombined with the Cμimmunoglobulin gene locus on chromosome 14 in several Burkitt lymphomas. The activation of the c-myconcogene by chromosome translocation implicates its involvement in B-cell oncogenesis.