First genetic linkage map of Taraxacum koksaghyz Rodin based on AFLP, SSR, COS and EST-SSR markers.
First genetic linkage map of Taraxacum koksaghyz Rodin based on AFLP, SSR, COS and EST-SSR markers.
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DOI：
10.1038/srep31031
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发表时间：
2016-08-04
影响因子：
4.6
通讯作者：
Ritter E
中科院分区：
文献类型：
--
作者：
Arias M;Hernandez M;Remondegui N;Huvenaars K;van Dijk P;Ritter E
蒲公英（Taraxacum koksaghyz Rodin，TKS）作为优质天然橡胶生产和菊粉生产的可能替代来源已在许多场合进行了研究。一些轮胎公司已经在测试TKS轮胎原型。还有许多关于从菊粉生产生物燃料和菊粉应用于改善健康和食品工业的研究。TKS的基因组资源有限，特别是在这个物种中没有遗传连锁图谱。利用AFLP、COS、SSR和EST-SSR标记构建了我国第一张TKS遗传连锁图谱。该连锁图包含来自亲本1的185个AFLP标记、来自亲本2的188个AFLP标记、75个通用AFLP标记、6个COS、1个SSR和63个EST-SSR标记。将EST-SSR序列与生菜的已知序列进行比对，可以将我们的TKS图谱与生菜图谱进行部分比对。对植物基因数据库的Blast搜索揭示了一些与下游应用有用基因的同源性。
Taraxacum koksaghyz Rodin (TKS) has been studied in many occasions as a possible alternative source for natural rubber production of good quality and for inulin production. Some tire companies are already testing TKS tire prototypes. There are also many investigations on the production of bio-fuels from inulin and inulin applications for health improvement and in the food industry. A limited amount of genomic resources exist for TKS and particularly no genetic linkage map is available in this species. We have constructed the first TKS genetic linkage map based on AFLP, COS, SSR and EST-SSR markers. The integrated linkage map with eight linkage groups (LG), representing the eight chromosomes of Russian dandelion, has 185 individual AFLP markers from parent 1, 188 individual AFLP markers from parent 2, 75 common AFLP markers and 6 COS, 1 SSR and 63 EST-SSR loci. Blasting the EST-SSR sequences against known sequences from lettuce allowed a partial alignment of our TKS map with a lettuce map. Blast searches against plant gene databases revealed some homologies with useful genes for downstream applications in the future.