Transcriptomic Insights into the Physiology of Aspergillus niger Approaching a Specific Growth Rate of Zero
Transcriptomic Insights into the Physiology of Aspergillus niger Approaching a Specific Growth Rate of Zero
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DOI：
10.1128/aem.00450-10
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发表时间：
2010-08-01
影响因子：
4.4
通讯作者：
Ram, Arthur F.
中科院分区：
文献类型：
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作者：
Jorgensen, Thomas R.;Nitsche, Benjamin M.;Ram, Arthur F.
丝状真菌在生长速率接近零时的生理学一直受到有限的研究和开发。为了使黑曲霉的生长与产物形成脱钩，我们重新审视并改进了尼日尔曲霉的retentostat培养方法。设计了一种新的保留装置，即使在高流速下也能实现可靠且几乎完全的细胞保留。转录组学分析用于探索在非常低的比生长速率下产物形成的潜力。碳和能量限制的retentostat培养物具有高度可重复性。虽然具体增长率接近于零（
The physiology of filamentous fungi at growth rates approaching zero has been subject to limited study and exploitation. With the aim of uncoupling product formation from growth, we have revisited and improved the retentostat cultivation method for Aspergillus niger. A new retention device was designed allowing reliable and nearly complete cell retention even at high flow rates. Transcriptomic analysis was used to explore the potential for product formation at very low specific growth rates. The carbon- and energy-limited retentostat cultures were highly reproducible. While the specific growth rate approached zero (